Live Cell Monitoring and Enrichment of Stem Cell-Derived β Cells Using Intracellular Zinc Content as a Population Marker

Jeffrey C. Davis*, Aharon Helman, José Rivera-Feliciano, Christine M. Langston, Elise N. Engquist, Douglas A. Melton

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Our laboratory and others have developed protocols to generate glucose-responsive stem cell–derived β cells in vitro. The cells resulting from these protocols could supplement or replace the use of human cadaveric islets for cell-based therapy for diabetes. The combination of an unlimited supply of pluripotent stem cell–derived β cells and gene-editing approaches will facilitate numerous in vitro studies not possible with cadaveric islets. Here, we describe a protocol for fluorescent labeling and isolation of stem cell–derived β cells. This purification of SC-β cells is based on intracellular zinc content and is a simple method to complement other approaches for generating and assaying these cells.

Original languageAmerican English
Article numbere99
JournalCurrent Protocols in Stem Cell Biology
Volume51
Issue number1
DOIs
StatePublished - 1 Dec 2019
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2019 The Authors.

Keywords

  • SC-β cell enrichment
  • intracellular zinc content
  • stem cell-derived β (SC-β) cell

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