TY - JOUR
T1 - Living Composites of Bacteria and Polymers as Biomimetic Films for Metal Sequestration and Bioremediation
AU - Knierim, Christian
AU - Enzeroth, Michaela
AU - Kaiser, Patrick
AU - Dams, Christian
AU - Nette, David
AU - Seubert, Andreas
AU - Klingl, Andreas
AU - Greenblatt, Charles L.
AU - Jérôme, Valérie
AU - Agarwal, Seema
AU - Freitag, Ruth
AU - Greiner, Andreas
N1 - Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Herein, we report on composite materials of biologically active microorganisms placed in a synthetic polymer matrix. These so-called "living composites" were utilized for gold sequestration (Micrococcus luteus) and bioremediation of nitrite (Nitrobacter winogradskyi) to demonstrate functionality. For the preparation of the living composites the bacteria were first encased in a water-soluble polymer fiber (poly(vinyl alcohol), PVA) followed by coating the fibers with a shell of hydrophobic poly(p-xylylene) (PPX) by chemical vapor deposition (CVD). The combination of bacteria with polymer materials assured the stability and biologically activity of the bacteria in an aqueous environment for several weeks. Water-insoluble core-shell fibers of poly(vinyl alcohol) (PVA) and poly(pxylylene) (PPX) containing M. luteus or N. winogradskyi are achieved by utilizing wet spinning in combination with CVD. The leaking of bacteria from the fibers is retarded in aqueous solution. The immobilized bacteria are used for sequestration of gold and degradation of nitrite from aqueous solutions. The biosorption of gold leads to encapsulated gold nanoparticles as analyzed by EDX, ICP-MS, and TEM.
AB - Herein, we report on composite materials of biologically active microorganisms placed in a synthetic polymer matrix. These so-called "living composites" were utilized for gold sequestration (Micrococcus luteus) and bioremediation of nitrite (Nitrobacter winogradskyi) to demonstrate functionality. For the preparation of the living composites the bacteria were first encased in a water-soluble polymer fiber (poly(vinyl alcohol), PVA) followed by coating the fibers with a shell of hydrophobic poly(p-xylylene) (PPX) by chemical vapor deposition (CVD). The combination of bacteria with polymer materials assured the stability and biologically activity of the bacteria in an aqueous environment for several weeks. Water-insoluble core-shell fibers of poly(vinyl alcohol) (PVA) and poly(pxylylene) (PPX) containing M. luteus or N. winogradskyi are achieved by utilizing wet spinning in combination with CVD. The leaking of bacteria from the fibers is retarded in aqueous solution. The immobilized bacteria are used for sequestration of gold and degradation of nitrite from aqueous solutions. The biosorption of gold leads to encapsulated gold nanoparticles as analyzed by EDX, ICP-MS, and TEM.
UR - http://www.scopus.com/inward/record.url?scp=84938998590&partnerID=8YFLogxK
U2 - 10.1002/mabi.201400538
DO - 10.1002/mabi.201400538
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C2 - 25845988
AN - SCOPUS:84938998590
SN - 1616-5187
VL - 15
SP - 1052
EP - 1059
JO - Macromolecular Bioscience
JF - Macromolecular Bioscience
IS - 8
ER -
