Abstract
We describe a novel procedure to load amphipathic weak acid molecules into preformed liposomes. Differences in calcium acetate concentrations across the liposomal membrane induce an increase of the internal pH. This pH imbalance serves as an efficient driving force to load and accumulate weak acids (5(6)-carboxyfluorescein and nalidixic acid) inside the lipid vesicles. The mechanism of loading and the relevance of the method in drug delivery systems are discussed.
Original language | English |
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Pages (from-to) | 257-265 |
Number of pages | 9 |
Journal | Biochimica et Biophysica Acta - Biomembranes |
Volume | 1240 |
Issue number | 2 |
DOIs | |
State | Published - 13 Dec 1995 |
Keywords
- 5(6)-Carboxyfluorescein
- Calcium acetate gradient
- Liposome
- Nalidixic acid
- pH gradient
- Remote loading