Loading of amphipathic weak acids into liposomes in response to transmembrane calcium acetate gradients

Stéphane Clerc*, Yechezkel Barenholz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

132 Scopus citations

Abstract

We describe a novel procedure to load amphipathic weak acid molecules into preformed liposomes. Differences in calcium acetate concentrations across the liposomal membrane induce an increase of the internal pH. This pH imbalance serves as an efficient driving force to load and accumulate weak acids (5(6)-carboxyfluorescein and nalidixic acid) inside the lipid vesicles. The mechanism of loading and the relevance of the method in drug delivery systems are discussed.

Original languageEnglish
Pages (from-to)257-265
Number of pages9
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1240
Issue number2
DOIs
StatePublished - 13 Dec 1995

Keywords

  • 5(6)-Carboxyfluorescein
  • Calcium acetate gradient
  • Liposome
  • Nalidixic acid
  • pH gradient
  • Remote loading

Fingerprint

Dive into the research topics of 'Loading of amphipathic weak acids into liposomes in response to transmembrane calcium acetate gradients'. Together they form a unique fingerprint.

Cite this