TY - JOUR
T1 - Local and peripheral cell‐mediated immune response to influenza virus in mice
AU - Shapira‐Nahor, Orit
AU - Zakay‐Rones, Zichria
PY - 1985/1
Y1 - 1985/1
N2 - Presentation of different influenza virus antigens generates different immune responses. Intranasal immunization with either live (VA) or formalin‐inactivated (VF) A/PR/8/34 (HoN1) influenza virus induced local as well as peripheral cell‐mediated immune response (CMI), as evidenced by elevation in 3H‐thymidine incorporation. Cell‐mediated immune response was detected as soon as 24–48 hr following the application of VA and 4–5 days following VF. Cell‐mediated immune response in both instances peaked on the 12th day and disappeared between 16 and 20 days after application. Local CMI response was threefold higher after immunization with VA (SI = 28.6) than with VF (SI = 9.4), while VF induced higher peripheral response (32.0 vs 17.7). The mononuclear cell population in the lungs increased, correlating with a rise in the stimulation index (SI). The percentage of IgA surface‐bearing B lymphocytes was significantly higher following IN administration of VA, but not following VF instillation. This corroborated the finding that VF failed to induce local antibody response in the lungs in spite of its capacity to stimulate humoral antibody and CMI responses. Mice immunized intramuscularly with both viral preparations developed a fair humoral antibody response without detectable CMI (peripheral or local).
AB - Presentation of different influenza virus antigens generates different immune responses. Intranasal immunization with either live (VA) or formalin‐inactivated (VF) A/PR/8/34 (HoN1) influenza virus induced local as well as peripheral cell‐mediated immune response (CMI), as evidenced by elevation in 3H‐thymidine incorporation. Cell‐mediated immune response was detected as soon as 24–48 hr following the application of VA and 4–5 days following VF. Cell‐mediated immune response in both instances peaked on the 12th day and disappeared between 16 and 20 days after application. Local CMI response was threefold higher after immunization with VA (SI = 28.6) than with VF (SI = 9.4), while VF induced higher peripheral response (32.0 vs 17.7). The mononuclear cell population in the lungs increased, correlating with a rise in the stimulation index (SI). The percentage of IgA surface‐bearing B lymphocytes was significantly higher following IN administration of VA, but not following VF instillation. This corroborated the finding that VF failed to induce local antibody response in the lungs in spite of its capacity to stimulate humoral antibody and CMI responses. Mice immunized intramuscularly with both viral preparations developed a fair humoral antibody response without detectable CMI (peripheral or local).
KW - CMI response
KW - humoral response
KW - mononuclear population
KW - Muenza virus vaccination
UR - http://www.scopus.com/inward/record.url?scp=0021930736&partnerID=8YFLogxK
U2 - 10.1002/jmv.1890150111
DO - 10.1002/jmv.1890150111
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C2 - 3871473
AN - SCOPUS:0021930736
SN - 0146-6615
VL - 15
SP - 81
EP - 91
JO - Journal of Medical Virology
JF - Journal of Medical Virology
IS - 1
ER -