Localization of pyruvate carboxylase in organic acid-producing Aspergillus strains

A. Bercovitz, Y. Peleg, E. Battat, J. S. Rokem, I. Goldberg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

The localization of pyruvate carboxylase (cytosolic or mitochondrial) was studied in nine different Aspergillus species (14 strains). In some species (A. aculeatus, A. flavus, A. foetidus, A. nidulans, A. ochraceus, and A. sojae), the pyruvate carboxylase activity could be detected only in the cystolic fraction of the cells. Pyruvate carboxylase has been found only in the mitochondrial fraction of two strains of Aspergillus wentii. In Aspergillus oryzae and in five strains of Aspergillus niger, pyruvate carboxylase activity was detected both in the mitochondrial fraction and in the cytosol. There was no quantitative or qualitative correlation between the activities of pyruvate carboxylase in the mitochondrial and cystolic fractions of the cells and the ability of the various Aspergillus strains to accumulate different organic acids.

Original languageEnglish
Pages (from-to)1594-1597
Number of pages4
JournalApplied and Environmental Microbiology
Volume56
Issue number6
DOIs
StatePublished - 1990

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