TY - JOUR
T1 - Localization of the binding site of an antibody affecting ATPase activity of chaperonin cpn60 from Bordetella pertussis
AU - Cejka, Zdenka
AU - Gould-Kostka, Jeanine
AU - Burns, Drusilla
AU - Kessel, Martin
PY - 1993
Y1 - 1993
N2 - Imaging processing has revealed the attachment site of antibody 54G8 on chaperonin 60 (cpn60) from Bordetella pertussis. This antibody, previously shown to affect the ability of chaperonin 10 (cpn10) to inhibit the ATPase activity of cpn60, is attached at the ends of the cpn60 and links the molecules into long chains. When only Fab fragments, which also affect ATPase activity, are used for labeling, these attach to both ends of the cpn60 molecule, but the long chains are not seen. Some perturbation of cpn60 was seen when Fab fragments were bound (Fab:cpn60 = 28:1).
AB - Imaging processing has revealed the attachment site of antibody 54G8 on chaperonin 60 (cpn60) from Bordetella pertussis. This antibody, previously shown to affect the ability of chaperonin 10 (cpn10) to inhibit the ATPase activity of cpn60, is attached at the ends of the cpn60 and links the molecules into long chains. When only Fab fragments, which also affect ATPase activity, are used for labeling, these attach to both ends of the cpn60 molecule, but the long chains are not seen. Some perturbation of cpn60 was seen when Fab fragments were bound (Fab:cpn60 = 28:1).
UR - http://www.scopus.com/inward/record.url?scp=0027500774&partnerID=8YFLogxK
U2 - 10.1006/jsbi.1993.1033
DO - 10.1006/jsbi.1993.1033
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AN - SCOPUS:0027500774
SN - 1047-8477
VL - 111
SP - 34
EP - 38
JO - Journal of Structural Biology
JF - Journal of Structural Biology
IS - 1
ER -