LogSpin: A simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues

Hila Yaffe, Kobi Buxdorf, Illil Shapira, Shachaf Ein-Gedi, Michal Moyal-Ben Zvi, Eyal Fridman, Menachem Moshelion, Maggie Levy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Abstract. Background: Rapid RNA extraction is commonly performed with commercial kits, which are very expensive and can involve toxic reagents. Most of these kits can be used with healthy plant tissues, but do not produce consistently high-quality RNA from necrotic fungus-infected tissues or fungal mycelium. Findings. We report on the development of a rapid and relatively inexpensive method for total RNA extraction from plants and fungus-infected tissues, as well as from insects and fungi, based on guanidine hydrochloride buffer and common DNA extraction columns originally used for the extraction and purification of plasmids and cosmids. Conclusions: The proposed method can be used reproducibly for RNA isolation from a variety of plant species. It can also be used with infected plant tissue and fungal mycelia, which are typically recalcitrant to standard nucleic acid extraction procedures.

Original languageAmerican English
Article number45
JournalBMC Research Notes
Volume5
DOIs
StatePublished - 2012

Bibliographical note

Funding Information:
The study was funded by research grant no. VWZN2556 from the Niedersachsen-Israel Fund to ML and by research grant no. IS-4210-09 to ML from the United States-Israel Binational Agricultural Research and Development Fund.

Keywords

  • Aphids
  • Fungus
  • Infected tissue
  • Plant
  • RNA extraction

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