Long-term restricted feeding alters circadian expression and reduces the level of inflammatory and disease markers

Hadas Sherman, Idan Frumin, Roee Gutman, Nava Chapnik, Axel Lorentz, Jenny Meylan, Johannes le Coutre, Oren Froy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

95 Scopus citations


The circadian clock in peripheral tissues can be entrained by restricted feeding (RF), a regimen that restricts the duration of food availability with no calorie restriction (CR). However, it is not known whether RF can delay the occurrence of age-associated changes similar to CR. We measured circadian expression of clock genes, disease marker genes, metabolic factors and inflammatory and allergy markers in mouse serum, liver, jejunum and white adipose tissue (WAT) after long-term RF of 4 months. We found that circadian rhythmicity is more robust and is phase advanced in most of the genes and proteins tested under RF. In addition, average daily levels of some disease and inflammatory markers were reduced under RF, including liverIl-6mRNA, tumour necrosis factor (TNF)-α and nuclear factor κB (NF-κB) protein; jejunumArginase, Afp, Gadd45β, Il-1α andIl-1β mRNA, and interleukin (IL)-6 and TNF-α protein and WATIl-6, Il-1β, Tnfα andNfκbmRNA. In contrast, the anti-inflammatory cytokineIl-10mRNA increased in the liver and jejunum. Our results suggest that RF may share some benefits with those of CR. As RF is a less harsh regimen to follow than CR, the data suggest it could be proposed for individuals seeking to improve their health.

Original languageAmerican English
Pages (from-to)2745-2759
Number of pages15
JournalJournal of Cellular and Molecular Medicine
Issue number12
StatePublished - Dec 2011


  • Circadian
  • Disease
  • Inflammation
  • Metabolism
  • Restricted feeding


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