Loss of WWOX Expression in Gastric Carcinoma

Rami I. Aqeilan*, Tamotsu Kuroki, Yuri Pekarsky, Omar Albagha, Francesco Trapasso, Raffaele Baffa, Kay Huebner, Pamela Edmonds, Carlo M. Croce

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

113 Scopus citations

Abstract

Purpose: WW domain-containing oxidoreductase (WWOX) is a tumor suppressor gene that maps to the common fragile site FRA16D on chromosome 16q23.3-24.1. To investigate the role of the WWOX gene in the development of gastric carcinoma, we examined a large series of primary adenocarcinomas and nine gastric cancer cell lines for the expression of Wwox. Experimental Design: Loss of heterozygosity, reverse-transcription-PCR, and immunohistochemistry were used to assess the role of WWOX in stomach cancer. A total of 81 primary gastric adenocarcinoma were analyzed. Results: Loss of heterozygosity was observed in 31% of the cases and loss of Wwox protein expression was found in 65% of gastric adenocarcinoma primary specimens and 33% of gastric cancer cell lines. In addition, we found a high correlation between Wwox and Fhit protein expression. Conclusions: Our results indicate that alterations of the WWOX gene may be involved quite frequently in gastric tumorigenesis. Our data could be used in future studies to develop diagnostic and targeted therapy of stomach cancer.

Original languageAmerican English
Pages (from-to)3053-3058
Number of pages6
JournalClinical Cancer Research
Volume10
Issue number9
DOIs
StatePublished - 1 May 2004
Externally publishedYes

Cite this