Low genetic differentiation among morphologically distinct Labeobarbus species (Teleostei: Cyprinidae) in the Lake Victoria and Albertine basins, Uganda: Insights from mitochondrial DNA

V. B. Muwanika, M. F. Nakamya, J. Rutaisire, B. Sivan, C. Masembe

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Labeobarbus altianalis and L. bynni bynni are hexaploid cyprinid fishes in the genus Labeobarbus. In the Great Lakes region of Africa, these two large-bodied barbs exhibit considerable morphological variations. Their intraspecific classification, currently based on geographical distribution and morphological variation, is of limited application due to the overlapping nature of the water systems. Patterns of genetic variation in the Albertine and Victoria drainage basins in Uganda were assessed using mitochondrial sequences of the control region (470 basepairs) and the cytochrome b gene (627 basepairs). At the control region 36 distinct haplotypes were observed in a total of 177 individuals. Relatively low overall nucleotide diversity (π = 0.010) was observed. Hierarchical analysis of the molecular variance revealed significant differentiation between populations in the total sample (FST = 0.172, p < 0.001), among populations within each basin (FSC = 0.047, p < 0.001), but no significant subdivision among basins (FCT = 0.131, p > 0.05). Phylogenetic analysis of the control region haplotypes resulted in a star-shaped phylogeny, with no clusters that reflect current taxonomic designation, morphotypes or basins. Phylogenetic analysis of cytochrome b gene sequences resulted in a tree topology similar to that observed for the control region. Thus the morphological characteristics separating the two species are intraspecific variation and the whole sample probably belongs to the same species.

Original languageAmerican English
Pages (from-to)143-153
Number of pages11
JournalAfrican Journal of Aquatic Science
Volume37
Issue number2
DOIs
StatePublished - Aug 2012

Bibliographical note

Funding Information:
Acknowledgements — Financial support for this study was provided through the Gender Mainstreaming Division by Sida-SAREC, administered by the School of Graduate Studies at Makerere University, and a CDR grant through the National Aquaculture Research and Development Centre, Kajjansi. We thank the field staff for their efforts in sample collection. The author group is interested in use of molecular tools to investigate phylogeography, population genetics and evolution of aquatic fauna in the Great Lakes region of Africa.

Keywords

  • Albertine basin
  • Barbus
  • Lake Victoria basin
  • genetic differentiation

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