Low occurrence of Bartonella in synanthropic mammals and associated ectoparasites in peri-urban areas from Central-Western and Southern Brazil

Luiz Ricardo Gonçalves, Shimon Harrus, Heitor Miraglia Herrera, Ricardo Gutiérrez, Daniela Pedrassani, Wesley Arruda Gimenes Nantes, Filipe Martins Santos, Grasiela Edith de Oliveira Porfírio, Wanessa Teixeira Gomes Barreto, Gabriel Carvalho de Macedo, William de Oliveira Assis, João Bosco Vilela Campos, Thiago Merighi Vieira da Silva, Juliano Biolchi, Keyla Carstens Marques de Sousa, Yaarit Nachum-Biala, Darci Moraes Barros-Battesti, Rosangela Zacarias Machado, Marcos Rogério André*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Worldwide, Bartonella species are known to infect a wide range of mammalian and arthropod hosts, including humans. The current study aimed to investigate the prevalence of Bartonella spp. in synanthropic mammals captured in peri-urban areas from Central-Western and Southern Brazil and their ectoparasites. For this aim, 160 mammals belonging to four species, and 218 associated arthropods were sampled. DNA was extracted and subjected to different Bartonella screening assays. Additionally, blood samples from 48 small rodents were submitted to liquid BAPGM culture followed by qPCR assay and solid culture. Two out of 55 Rattus captured in Santa Catarina state were PCR-positive for Bartonella when targeting the nuoG, 16S, and ITS loci. Sequences showed high homology with Bartonella coopersplainsensis. Conversely, all 48 small rodents, 14 capybaras and 43 opossum DNA samples from animals trapped in Mato Grosso do Sul were Bartonella negative in the HRM real time PCR assays targeting the ITS locus and gltA gene. Additionally, all mammal-associated ectoparasites showed negativity results based on HRM real time PCR assays. The present study showed, for the first time, the occurrence of B. coopersplainsensis in Brazil, shedding some light on the distribution of rats-related Bartonella in South America. In addition, the majority of rodents and marsupials were negative for Bartonella spp. Since B. coopersplainsensis reservoirs - Rattus spp. - are widely dispersed around the globe, their zoonotic potential should be further investigated.

Original languageAmerican English
Article number105513
JournalActa Tropica
Volume207
DOIs
StatePublished - Jul 2020

Bibliographical note

Publisher Copyright:
© 2020 Elsevier B.V.

Keywords

  • BAPGM
  • Bartonella coopersplainsensis
  • capybaras
  • culture
  • fleas
  • lice
  • opossums
  • rodents
  • ticks

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