Low occurrence of Bartonella in synanthropic mammals and associated ectoparasites in peri-urban areas from Central-Western and Southern Brazil

Luiz Ricardo Gonçalves; Shimon Harrus; Heitor Miraglia Herrera; Ricardo Gutiérrez; Daniela Pedrassani; Wesley Arruda Gimenes Nantes; Filipe Martins Santos; Grasiela Edith de Oliveira Porfírio; Wanessa Teixeira Gomes Barreto; Gabriel Carvalho de Macedo; William de Oliveira Assis; João Bosco Vilela Campos; Thiago Merighi Vieira da Silva; Juliano Biolchi; Keyla Carstens Marques de Sousa; Yaarit Nachum-Biala; Darci Moraes Barros-Battesti; Rosangela Zacarias Machado; Marcos Rogério André

doi:10.1016/j.actatropica.2020.105513

Low occurrence of Bartonella in synanthropic mammals and associated ectoparasites in peri-urban areas from Central-Western and Southern Brazil

Luiz Ricardo Gonçalves, Shimon Harrus, Heitor Miraglia Herrera, Ricardo Gutiérrez, Daniela Pedrassani, Wesley Arruda Gimenes Nantes, Filipe Martins Santos, Grasiela Edith de Oliveira Porfírio, Wanessa Teixeira Gomes Barreto, Gabriel Carvalho de Macedo, William de Oliveira Assis, João Bosco Vilela Campos, Thiago Merighi Vieira da Silva, Juliano Biolchi, Keyla Carstens Marques de Sousa, Yaarit Nachum-Biala, Darci Moraes Barros-Battesti, Rosangela Zacarias Machado, Marcos Rogério André^*

^*Corresponding author for this work

The Koret School of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Worldwide, Bartonella species are known to infect a wide range of mammalian and arthropod hosts, including humans. The current study aimed to investigate the prevalence of Bartonella spp. in synanthropic mammals captured in peri-urban areas from Central-Western and Southern Brazil and their ectoparasites. For this aim, 160 mammals belonging to four species, and 218 associated arthropods were sampled. DNA was extracted and subjected to different Bartonella screening assays. Additionally, blood samples from 48 small rodents were submitted to liquid BAPGM culture followed by qPCR assay and solid culture. Two out of 55 Rattus captured in Santa Catarina state were PCR-positive for Bartonella when targeting the nuoG, 16S, and ITS loci. Sequences showed high homology with Bartonella coopersplainsensis. Conversely, all 48 small rodents, 14 capybaras and 43 opossum DNA samples from animals trapped in Mato Grosso do Sul were Bartonella negative in the HRM real time PCR assays targeting the ITS locus and gltA gene. Additionally, all mammal-associated ectoparasites showed negativity results based on HRM real time PCR assays. The present study showed, for the first time, the occurrence of B. coopersplainsensis in Brazil, shedding some light on the distribution of rats-related Bartonella in South America. In addition, the majority of rodents and marsupials were negative for Bartonella spp. Since B. coopersplainsensis reservoirs - Rattus spp. - are widely dispersed around the globe, their zoonotic potential should be further investigated.

Original language	American English
Article number	105513
Journal	Acta Tropica
Volume	207
DOIs	https://doi.org/10.1016/j.actatropica.2020.105513
State	Published - Jul 2020

Bibliographical note

Funding Information:
To the Graduate Program of Agricultural and Livestock Microbiology ( Universidade Estadual Paulista , UNESP, Campus Jaboticabal), the FAPESP (Foundation for Research Support of the State of São Paulo - Process 2018/02753-0), FUNDECT (Foundation for Support to the Development of Education, Science and Technology of the State of Mato Grosso do Sul, Case 59 / 300.187 / 2016) and CNPq for the Productivity Grant granted to the MRA (CNPq Process No. 302420 / 2017-7). The present study was carried out with the support of the Coordination for the Improvement of Higher Education (CAPES - Code 1). L.R.G. received a scholarship from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.

Publisher Copyright:
© 2020 Elsevier B.V.

Keywords

BAPGM
Bartonella coopersplainsensis
capybaras
culture
fleas
lice
opossums
rodents
ticks

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.actatropica.2020.105513

Cite this

Gonçalves, L. R., Harrus, S., Herrera, H. M., Gutiérrez, R., Pedrassani, D., Nantes, W. A. G., Santos, F. M., Porfírio, G. E. D. O., Barreto, W. T. G., de Macedo, G. C., Assis, W. D. O., Campos, J. B. V., da Silva, T. M. V., Biolchi, J., de Sousa, K. C. M., Nachum-Biala, Y., Barros-Battesti, D. M., Machado, R. Z., & André, M. R. (2020). Low occurrence of Bartonella in synanthropic mammals and associated ectoparasites in peri-urban areas from Central-Western and Southern Brazil. Acta Tropica, 207, Article 105513. https://doi.org/10.1016/j.actatropica.2020.105513

@article{baf67dad7eed409a9889a1e55c9f76d0,

title = "Low occurrence of Bartonella in synanthropic mammals and associated ectoparasites in peri-urban areas from Central-Western and Southern Brazil",

abstract = "Worldwide, Bartonella species are known to infect a wide range of mammalian and arthropod hosts, including humans. The current study aimed to investigate the prevalence of Bartonella spp. in synanthropic mammals captured in peri-urban areas from Central-Western and Southern Brazil and their ectoparasites. For this aim, 160 mammals belonging to four species, and 218 associated arthropods were sampled. DNA was extracted and subjected to different Bartonella screening assays. Additionally, blood samples from 48 small rodents were submitted to liquid BAPGM culture followed by qPCR assay and solid culture. Two out of 55 Rattus captured in Santa Catarina state were PCR-positive for Bartonella when targeting the nuoG, 16S, and ITS loci. Sequences showed high homology with Bartonella coopersplainsensis. Conversely, all 48 small rodents, 14 capybaras and 43 opossum DNA samples from animals trapped in Mato Grosso do Sul were Bartonella negative in the HRM real time PCR assays targeting the ITS locus and gltA gene. Additionally, all mammal-associated ectoparasites showed negativity results based on HRM real time PCR assays. The present study showed, for the first time, the occurrence of B. coopersplainsensis in Brazil, shedding some light on the distribution of rats-related Bartonella in South America. In addition, the majority of rodents and marsupials were negative for Bartonella spp. Since B. coopersplainsensis reservoirs - Rattus spp. - are widely dispersed around the globe, their zoonotic potential should be further investigated.",

keywords = "BAPGM, Bartonella coopersplainsensis, capybaras, culture, fleas, lice, opossums, rodents, ticks",

author = "Gon{\c c}alves, {Luiz Ricardo} and Shimon Harrus and Herrera, {Heitor Miraglia} and Ricardo Guti{\'e}rrez and Daniela Pedrassani and Nantes, {Wesley Arruda Gimenes} and Santos, {Filipe Martins} and Porf{\'i}rio, {Grasiela Edith de Oliveira} and Barreto, {Wanessa Teixeira Gomes} and {de Macedo}, {Gabriel Carvalho} and Assis, {William de Oliveira} and Campos, {Jo{\~a}o Bosco Vilela} and {da Silva}, {Thiago Merighi Vieira} and Juliano Biolchi and {de Sousa}, {Keyla Carstens Marques} and Yaarit Nachum-Biala and Barros-Battesti, {Darci Moraes} and Machado, {Rosangela Zacarias} and Andr{\'e}, {Marcos Rog{\'e}rio}",

note = "Funding Information: To the Graduate Program of Agricultural and Livestock Microbiology ( Universidade Estadual Paulista , UNESP, Campus Jaboticabal), the FAPESP (Foundation for Research Support of the State of S{\~a}o Paulo - Process 2018/02753-0), FUNDECT (Foundation for Support to the Development of Education, Science and Technology of the State of Mato Grosso do Sul, Case 59 / 300.187 / 2016) and CNPq for the Productivity Grant granted to the MRA (CNPq Process No. 302420 / 2017-7). The present study was carried out with the support of the Coordination for the Improvement of Higher Education (CAPES - Code 1). L.R.G. received a scholarship from the Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

year = "2020",

month = jul,

doi = "10.1016/j.actatropica.2020.105513",

language = "American English",

volume = "207",

journal = "Acta Tropica",

issn = "0001-706X",

publisher = "Elsevier",

}

Gonçalves, LR, Harrus, S, Herrera, HM, Gutiérrez, R, Pedrassani, D, Nantes, WAG, Santos, FM, Porfírio, GEDO, Barreto, WTG, de Macedo, GC, Assis, WDO, Campos, JBV, da Silva, TMV, Biolchi, J, de Sousa, KCM, Nachum-Biala, Y, Barros-Battesti, DM, Machado, RZ & André, MR 2020, 'Low occurrence of Bartonella in synanthropic mammals and associated ectoparasites in peri-urban areas from Central-Western and Southern Brazil', Acta Tropica, vol. 207, 105513. https://doi.org/10.1016/j.actatropica.2020.105513

TY - JOUR

T1 - Low occurrence of Bartonella in synanthropic mammals and associated ectoparasites in peri-urban areas from Central-Western and Southern Brazil

AU - Gonçalves, Luiz Ricardo

AU - Harrus, Shimon

AU - Herrera, Heitor Miraglia

AU - Gutiérrez, Ricardo

AU - Pedrassani, Daniela

AU - Nantes, Wesley Arruda Gimenes

AU - Santos, Filipe Martins

AU - Porfírio, Grasiela Edith de Oliveira

AU - Barreto, Wanessa Teixeira Gomes

AU - de Macedo, Gabriel Carvalho

AU - Assis, William de Oliveira

AU - Campos, João Bosco Vilela

AU - da Silva, Thiago Merighi Vieira

AU - Biolchi, Juliano

AU - de Sousa, Keyla Carstens Marques

AU - Nachum-Biala, Yaarit

AU - Barros-Battesti, Darci Moraes

AU - Machado, Rosangela Zacarias

AU - André, Marcos Rogério

N1 - Funding Information: To the Graduate Program of Agricultural and Livestock Microbiology ( Universidade Estadual Paulista , UNESP, Campus Jaboticabal), the FAPESP (Foundation for Research Support of the State of São Paulo - Process 2018/02753-0), FUNDECT (Foundation for Support to the Development of Education, Science and Technology of the State of Mato Grosso do Sul, Case 59 / 300.187 / 2016) and CNPq for the Productivity Grant granted to the MRA (CNPq Process No. 302420 / 2017-7). The present study was carried out with the support of the Coordination for the Improvement of Higher Education (CAPES - Code 1). L.R.G. received a scholarship from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior. Publisher Copyright: © 2020 Elsevier B.V.

PY - 2020/7

Y1 - 2020/7

N2 - Worldwide, Bartonella species are known to infect a wide range of mammalian and arthropod hosts, including humans. The current study aimed to investigate the prevalence of Bartonella spp. in synanthropic mammals captured in peri-urban areas from Central-Western and Southern Brazil and their ectoparasites. For this aim, 160 mammals belonging to four species, and 218 associated arthropods were sampled. DNA was extracted and subjected to different Bartonella screening assays. Additionally, blood samples from 48 small rodents were submitted to liquid BAPGM culture followed by qPCR assay and solid culture. Two out of 55 Rattus captured in Santa Catarina state were PCR-positive for Bartonella when targeting the nuoG, 16S, and ITS loci. Sequences showed high homology with Bartonella coopersplainsensis. Conversely, all 48 small rodents, 14 capybaras and 43 opossum DNA samples from animals trapped in Mato Grosso do Sul were Bartonella negative in the HRM real time PCR assays targeting the ITS locus and gltA gene. Additionally, all mammal-associated ectoparasites showed negativity results based on HRM real time PCR assays. The present study showed, for the first time, the occurrence of B. coopersplainsensis in Brazil, shedding some light on the distribution of rats-related Bartonella in South America. In addition, the majority of rodents and marsupials were negative for Bartonella spp. Since B. coopersplainsensis reservoirs - Rattus spp. - are widely dispersed around the globe, their zoonotic potential should be further investigated.

AB - Worldwide, Bartonella species are known to infect a wide range of mammalian and arthropod hosts, including humans. The current study aimed to investigate the prevalence of Bartonella spp. in synanthropic mammals captured in peri-urban areas from Central-Western and Southern Brazil and their ectoparasites. For this aim, 160 mammals belonging to four species, and 218 associated arthropods were sampled. DNA was extracted and subjected to different Bartonella screening assays. Additionally, blood samples from 48 small rodents were submitted to liquid BAPGM culture followed by qPCR assay and solid culture. Two out of 55 Rattus captured in Santa Catarina state were PCR-positive for Bartonella when targeting the nuoG, 16S, and ITS loci. Sequences showed high homology with Bartonella coopersplainsensis. Conversely, all 48 small rodents, 14 capybaras and 43 opossum DNA samples from animals trapped in Mato Grosso do Sul were Bartonella negative in the HRM real time PCR assays targeting the ITS locus and gltA gene. Additionally, all mammal-associated ectoparasites showed negativity results based on HRM real time PCR assays. The present study showed, for the first time, the occurrence of B. coopersplainsensis in Brazil, shedding some light on the distribution of rats-related Bartonella in South America. In addition, the majority of rodents and marsupials were negative for Bartonella spp. Since B. coopersplainsensis reservoirs - Rattus spp. - are widely dispersed around the globe, their zoonotic potential should be further investigated.

KW - BAPGM

KW - Bartonella coopersplainsensis

KW - capybaras

KW - culture

KW - fleas

KW - lice

KW - opossums

KW - rodents

KW - ticks

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U2 - 10.1016/j.actatropica.2020.105513

DO - 10.1016/j.actatropica.2020.105513

M3 - Article

C2 - 32371220

AN - SCOPUS:85086886211

SN - 0001-706X

VL - 207

JO - Acta Tropica

JF - Acta Tropica

M1 - 105513

ER -

Low occurrence of Bartonella in synanthropic mammals and associated ectoparasites in peri-urban areas from Central-Western and Southern Brazil

Abstract

Bibliographical note

Keywords

UN SDGs

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