Worldwide, Bartonella species are known to infect a wide range of mammalian and arthropod hosts, including humans. The current study aimed to investigate the prevalence of Bartonella spp. in synanthropic mammals captured in peri-urban areas from Central-Western and Southern Brazil and their ectoparasites. For this aim, 160 mammals belonging to four species, and 218 associated arthropods were sampled. DNA was extracted and subjected to different Bartonella screening assays. Additionally, blood samples from 48 small rodents were submitted to liquid BAPGM culture followed by qPCR assay and solid culture. Two out of 55 Rattus captured in Santa Catarina state were PCR-positive for Bartonella when targeting the nuoG, 16S, and ITS loci. Sequences showed high homology with Bartonella coopersplainsensis. Conversely, all 48 small rodents, 14 capybaras and 43 opossum DNA samples from animals trapped in Mato Grosso do Sul were Bartonella negative in the HRM real time PCR assays targeting the ITS locus and gltA gene. Additionally, all mammal-associated ectoparasites showed negativity results based on HRM real time PCR assays. The present study showed, for the first time, the occurrence of B. coopersplainsensis in Brazil, shedding some light on the distribution of rats-related Bartonella in South America. In addition, the majority of rodents and marsupials were negative for Bartonella spp. Since B. coopersplainsensis reservoirs - Rattus spp. - are widely dispersed around the globe, their zoonotic potential should be further investigated.
Bibliographical noteFunding Information:
To the Graduate Program of Agricultural and Livestock Microbiology ( Universidade Estadual Paulista , UNESP, Campus Jaboticabal), the FAPESP (Foundation for Research Support of the State of São Paulo - Process 2018/02753-0), FUNDECT (Foundation for Support to the Development of Education, Science and Technology of the State of Mato Grosso do Sul, Case 59 / 300.187 / 2016) and CNPq for the Productivity Grant granted to the MRA (CNPq Process No. 302420 / 2017-7). The present study was carried out with the support of the Coordination for the Improvement of Higher Education (CAPES - Code 1). L.R.G. received a scholarship from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
© 2020 Elsevier B.V.
- Bartonella coopersplainsensis