Abstract
New techniques in laser Raman spectroscopy are used to obtain spectra of aqueous solutions of lysozylme for frequency shifts as small as 5 cm−1. In addition, Raman measurements are made on two crystalline forms of hen egg white lysozyme. The spectra obtained from the solution and from the crystal are found to be similar for frequencies above 100 cm−1. However, a low‐frequency band at 25 cm−1 observed in crystalline lysozyme is not found in the solution, indicating that this band cannot be attributed to an internal molecular vibration.
Original language | English |
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Pages (from-to) | 219-225 |
Number of pages | 7 |
Journal | Biopolymers |
Volume | 15 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1976 |
Externally published | Yes |