LuxR solos in the plant endophyte kosakonia sp. Strain KO348

Susan Mosquito; Xianfa Meng; Giulia Devescovi; Iris Bertani; Alexander M. Geller; Asaf Levy; Michael P. Myers; Cristina Bez; Sonia Covaceuszach; Vittorio Venturi

doi:10.1128/AEM.00622-20

LuxR solos in the plant endophyte kosakonia sp. Strain KO348

Susan Mosquito, Xianfa Meng, Giulia Devescovi, Iris Bertani, Alexander M. Geller, Asaf Levy, Michael P. Myers, Cristina Bez, Sonia Covaceuszach, Vittorio Venturi^*

^*Corresponding author for this work

Department of Plant Pathology and Microbiology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Endophytes are microorganisms that live inside plants and are often beneficial for the host. Kosakonia is a novel bacterial genus that includes several species that are diazotrophic and plant associated. This study revealed two quorum sensing-related LuxR solos, designated LoxR and PsrR, in the plant endophyte Kosakonia sp. strain KO348. LoxR modeling and biochemical studies demonstrated that LoxR binds N-acyl homoserine lactones (AHLs) in a promiscuous way. PsrR, on the other hand, belongs to the subfamily of plant-associated-bacterium (PAB) LuxR solos that respond to plant compounds. Target promoter studies as well as modeling and phylogenetic comparisons suggest that PAB LuxR solos are likely to respond to different plant compounds. Finally, LoxR is involved in the regulation of T6SS and PsrR plays a role in root endosphere colonization.

Original language	English
Article number	e00622-20
Journal	Applied and Environmental Microbiology
Volume	86
Issue number	13
DOIs	https://doi.org/10.1128/AEM.00622-20
State	Published - 1 Jul 2020

Bibliographical note

Publisher Copyright:
© 2020 Mosquito et al.

Keywords

Bacteria
Cell-cell signaling
Endophyte
Gene regulation
LuxR solos

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10.1128/AEM.00622-20

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title = "LuxR solos in the plant endophyte kosakonia sp. Strain KO348",

abstract = "Endophytes are microorganisms that live inside plants and are often beneficial for the host. Kosakonia is a novel bacterial genus that includes several species that are diazotrophic and plant associated. This study revealed two quorum sensing-related LuxR solos, designated LoxR and PsrR, in the plant endophyte Kosakonia sp. strain KO348. LoxR modeling and biochemical studies demonstrated that LoxR binds N-acyl homoserine lactones (AHLs) in a promiscuous way. PsrR, on the other hand, belongs to the subfamily of plant-associated-bacterium (PAB) LuxR solos that respond to plant compounds. Target promoter studies as well as modeling and phylogenetic comparisons suggest that PAB LuxR solos are likely to respond to different plant compounds. Finally, LoxR is involved in the regulation of T6SS and PsrR plays a role in root endosphere colonization.",

keywords = "Bacteria, Cell-cell signaling, Endophyte, Gene regulation, LuxR solos",

author = "Susan Mosquito and Xianfa Meng and Giulia Devescovi and Iris Bertani and Geller, {Alexander M.} and Asaf Levy and Myers, {Michael P.} and Cristina Bez and Sonia Covaceuszach and Vittorio Venturi",

note = "Publisher Copyright: {\textcopyright} 2020 Mosquito et al.",

year = "2020",

month = jul,

day = "1",

doi = "10.1128/AEM.00622-20",

language = "אנגלית",

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AU - Mosquito, Susan

AU - Meng, Xianfa

AU - Devescovi, Giulia

AU - Bertani, Iris

AU - Geller, Alexander M.

AU - Levy, Asaf

AU - Myers, Michael P.

AU - Bez, Cristina

AU - Covaceuszach, Sonia

AU - Venturi, Vittorio

PY - 2020/7/1

Y1 - 2020/7/1

N2 - Endophytes are microorganisms that live inside plants and are often beneficial for the host. Kosakonia is a novel bacterial genus that includes several species that are diazotrophic and plant associated. This study revealed two quorum sensing-related LuxR solos, designated LoxR and PsrR, in the plant endophyte Kosakonia sp. strain KO348. LoxR modeling and biochemical studies demonstrated that LoxR binds N-acyl homoserine lactones (AHLs) in a promiscuous way. PsrR, on the other hand, belongs to the subfamily of plant-associated-bacterium (PAB) LuxR solos that respond to plant compounds. Target promoter studies as well as modeling and phylogenetic comparisons suggest that PAB LuxR solos are likely to respond to different plant compounds. Finally, LoxR is involved in the regulation of T6SS and PsrR plays a role in root endosphere colonization.

AB - Endophytes are microorganisms that live inside plants and are often beneficial for the host. Kosakonia is a novel bacterial genus that includes several species that are diazotrophic and plant associated. This study revealed two quorum sensing-related LuxR solos, designated LoxR and PsrR, in the plant endophyte Kosakonia sp. strain KO348. LoxR modeling and biochemical studies demonstrated that LoxR binds N-acyl homoserine lactones (AHLs) in a promiscuous way. PsrR, on the other hand, belongs to the subfamily of plant-associated-bacterium (PAB) LuxR solos that respond to plant compounds. Target promoter studies as well as modeling and phylogenetic comparisons suggest that PAB LuxR solos are likely to respond to different plant compounds. Finally, LoxR is involved in the regulation of T6SS and PsrR plays a role in root endosphere colonization.

KW - Bacteria

KW - Cell-cell signaling

KW - Endophyte

KW - Gene regulation

KW - LuxR solos

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ER -

LuxR solos in the plant endophyte kosakonia sp. Strain KO348

Abstract

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Keywords

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