Lysine 27 of histone H3.3 is a fine modulator of developmental gene expression and stands as an epigenetic checkpoint for lignin biosynthesis in Arabidopsis

Kateryna Fal, Alexandre Berr, Marie Le Masson, Adi Faigenboim, Emeline Pano, Nickolay Ishkhneli, Netta Lee Moyal, Claire Villette, Denisa Tomkova, Marie Edith Chabouté, Leor Eshed Williams, Cristel C. Carles*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Chromatin is a dynamic platform within which gene expression is controlled by epigenetic modifications, notably targeting amino acid residues of histone H3. Among them is lysine 27 of H3 (H3K27), the trimethylation of which by the Polycomb Repressive Complex 2 (PRC2) is instrumental in regulating spatiotemporal patterns of key developmental genes. H3K27 is also subjected to acetylation and is found at sites of active transcription. Most information on the function of histone residues and their associated modifications in plants was obtained from studies of loss-of-function mutants for the complexes that modify them. To decrypt the genuine function of H3K27, we expressed a non-modifiable variant of H3 at residue K27 (H3.3K27A) in Arabidopsis, and developed a multi-scale approach combining in-depth phenotypical and cytological analyses, with transcriptomics and metabolomics. We uncovered that the H3.3K27A variant causes severe developmental defects, part of them are reminiscent of PRC2 mutants, part of them are new. They include early flowering, increased callus formation and short stems with thicker xylem cell layer. This latest phenotype correlates with mis-regulation of phenylpropanoid biosynthesis. Overall, our results reveal novel roles of H3K27 in plant cell fates and metabolic pathways, and highlight an epigenetic control point for elongation and lignin composition of the stem.

Original languageAmerican English
Pages (from-to)1085-1100
Number of pages16
JournalNew Phytologist
Volume238
Issue number3
DOIs
StatePublished - May 2023

Bibliographical note

Publisher Copyright:
© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.

Keywords

  • development
  • flowering
  • gene expression
  • histone 3
  • lysine residue
  • methylation
  • tissue regeneration
  • xylem

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