Macrophages promote development of human erythroid precursors in transferrin-free culture medium

J. M. Leimberg, A. M. Konijn, E. Fibach*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Iron is mandatory for cell growth and development. Erythroid precursors need iron to a greater degree for hemoglobinization. Culturing erythroid precursors under serum and transferrin-free conditions resulted in their death, whereas under the same conditions, but in the presence of macrophages erythroid cell growth and development was evident as measured by hemoglobin (Hb)-specific cytochemical staining, flow cytometric immuno-staining of glycophorin A and Hb quantitation by a spectrophotometric method as well as by high performance liquid chromatography. Thus, macrophages support erythroid cell growth in the absence of transferrin, most likely by providing iron.

Original languageEnglish
Pages (from-to)73-76
Number of pages4
JournalHematology
Volume10
Issue number1
DOIs
StatePublished - Feb 2005

Keywords

  • Erythroid precursors
  • Ferritin
  • Iron
  • Macrophages
  • Transferrin

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