Abstract
Iron is mandatory for cell growth and development. Erythroid precursors need iron to a greater degree for hemoglobinization. Culturing erythroid precursors under serum and transferrin-free conditions resulted in their death, whereas under the same conditions, but in the presence of macrophages erythroid cell growth and development was evident as measured by hemoglobin (Hb)-specific cytochemical staining, flow cytometric immuno-staining of glycophorin A and Hb quantitation by a spectrophotometric method as well as by high performance liquid chromatography. Thus, macrophages support erythroid cell growth in the absence of transferrin, most likely by providing iron.
Original language | English |
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Pages (from-to) | 73-76 |
Number of pages | 4 |
Journal | Hematology |
Volume | 10 |
Issue number | 1 |
DOIs | |
State | Published - Feb 2005 |
Keywords
- Erythroid precursors
- Ferritin
- Iron
- Macrophages
- Transferrin