Manipulations of cholinesterase gene expression modulate murine megakaryocytopoiesis in vitro

Deborah Patinkin; Shlomo Seidman; Fritz Eckstein; Fritz Benseler; Haim Zakut; Hermona Soreq

Manipulations of cholinesterase gene expression modulate murine megakaryocytopoiesis in vitro

Deborah Patinkin^*
, Shlomo Seidman
, Fritz Eckstein
, Fritz Benseler
, Haim Zakut
, Hermona Soreq

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Megakaryocytopoiesis was selectively inhibited in cultured murine bone marrow cells by a 15-mer oligodeoxynucleotide complementary to the initiator AUG region in butyrylcholinesterase mRNA. Furthermore, conditioned medium from Xenopus oocytes producing recombinant butyrylcholinesterase stimulated megakaryocytopoiesis. These observations implicate butyrylcholinesterase in megakaryocytopoiesis and suggest application of oligodeoxynucleotides for modulating bone marrow development.

Original language	English
Pages (from-to)	6046-6050
Number of pages	5
Journal	Molecular and Cellular Biology
Volume	10
Issue number	11
State	Published - 1990

Cite this

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title = "Manipulations of cholinesterase gene expression modulate murine megakaryocytopoiesis in vitro",

abstract = "Megakaryocytopoiesis was selectively inhibited in cultured murine bone marrow cells by a 15-mer oligodeoxynucleotide complementary to the initiator AUG region in butyrylcholinesterase mRNA. Furthermore, conditioned medium from Xenopus oocytes producing recombinant butyrylcholinesterase stimulated megakaryocytopoiesis. These observations implicate butyrylcholinesterase in megakaryocytopoiesis and suggest application of oligodeoxynucleotides for modulating bone marrow development.",

author = "Deborah Patinkin and Shlomo Seidman and Fritz Eckstein and Fritz Benseler and Haim Zakut and Hermona Soreq",

year = "1990",

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AU - Patinkin, Deborah

AU - Seidman, Shlomo

AU - Eckstein, Fritz

AU - Benseler, Fritz

AU - Zakut, Haim

AU - Soreq, Hermona

PY - 1990

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N2 - Megakaryocytopoiesis was selectively inhibited in cultured murine bone marrow cells by a 15-mer oligodeoxynucleotide complementary to the initiator AUG region in butyrylcholinesterase mRNA. Furthermore, conditioned medium from Xenopus oocytes producing recombinant butyrylcholinesterase stimulated megakaryocytopoiesis. These observations implicate butyrylcholinesterase in megakaryocytopoiesis and suggest application of oligodeoxynucleotides for modulating bone marrow development.

AB - Megakaryocytopoiesis was selectively inhibited in cultured murine bone marrow cells by a 15-mer oligodeoxynucleotide complementary to the initiator AUG region in butyrylcholinesterase mRNA. Furthermore, conditioned medium from Xenopus oocytes producing recombinant butyrylcholinesterase stimulated megakaryocytopoiesis. These observations implicate butyrylcholinesterase in megakaryocytopoiesis and suggest application of oligodeoxynucleotides for modulating bone marrow development.

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C2 - 2233731

AN - SCOPUS:0025175718

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JF - Molecular and Cellular Biology

IS - 11

ER -

Manipulations of cholinesterase gene expression modulate murine megakaryocytopoiesis in vitro

Abstract

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