MAP-quest: Could we produce constitutively active variants of MAP kinases?

Nadav Askari, Ron Diskin, Michal Avitzour, Gilad Yaakov, Oded Livnah, David Engelberg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Constitutively active mutants that acquired intrinsic activity and escaped regulation, serve as powerful tools for revealing the biochemical, biological and pathological functions of proteins. Such mutants are not available for mitogen-activated protein kinases (MAPKs). It is not known how to mimic the unusual mode of MAPK activation and to enforce, by mutations, their active conformation. In this review we describe the strategies employed in attempts to overcome this obstacle. We focus on a recent breakthrough with the p38 family that suggests that active variants of all MAPKs will soon be available.

Original languageAmerican English
Pages (from-to)231-240
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume252
Issue number1-2
DOIs
StatePublished - 27 Jun 2006

Keywords

  • ERK
  • Gain-of-function mutations
  • HOG1
  • JNK
  • MAP kinase
  • p38

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