TY - JOUR
T1 - Mapping and introgression of a tomato yellow leaf curl virus tolerance gene, TY-1
AU - Zamir, D.
AU - Ekstein-Michelson, I.
AU - Zakay, Y.
AU - Navot, N.
AU - Zeidan, M.
AU - Sarfatti, M.
AU - Eshed, Y.
AU - Harel, E.
AU - Pleban, T.
AU - van-Oss, H.
AU - Kedar, N.
AU - Rabinowitch, H. D.
AU - Czosnek, H.
PY - 1994/5
Y1 - 1994/5
N2 - The whitefly-transmitted tomato yellow-leaf curl gemini-virus (TYLCV) is a major pathogen of tomatoes. The wild tomato species Lycopersicon chilense, which is resistant to the virus, was crossed to the cultivated tomato, L. esculentum. The backcross-1 selfed (BC1S1) generation was inoculated and a symptomless plant was selected. This plant was analyzed using 61 molecular markers, which span the tomato genome, to determine which L. chilense chromosome segments were introgressed. A BC2S1 population was cage-inoculated with viroliferous whiteflies (Bemisia tabaci), the natural insect vector of the virus, and subjected to RFLP analysis. Markers on chromosomes 3 and 6 were significantly associated with the level of tolerance; the association of chromosome-6 markers was further substantiated in two additional BC2S1 populations. A tolerant BC2S1 plant which was homozygous for L. chilense introgressions in chromosomes 3, 6 and 7 was crossed to generate a BC3S1 population which was planted in an infested field. A TYLCV-tolerance gene with partial dominance, TY-1, was mapped to chromosome 6; two modifier genes were mapped to chromosomes 3 and 7. Field and whitefly-mediated cage inoculations of nearly-isogenic lines in BC3S3 supported our conclusion that TY-1 is the major TYLCV-tolerance locus.
AB - The whitefly-transmitted tomato yellow-leaf curl gemini-virus (TYLCV) is a major pathogen of tomatoes. The wild tomato species Lycopersicon chilense, which is resistant to the virus, was crossed to the cultivated tomato, L. esculentum. The backcross-1 selfed (BC1S1) generation was inoculated and a symptomless plant was selected. This plant was analyzed using 61 molecular markers, which span the tomato genome, to determine which L. chilense chromosome segments were introgressed. A BC2S1 population was cage-inoculated with viroliferous whiteflies (Bemisia tabaci), the natural insect vector of the virus, and subjected to RFLP analysis. Markers on chromosomes 3 and 6 were significantly associated with the level of tolerance; the association of chromosome-6 markers was further substantiated in two additional BC2S1 populations. A tolerant BC2S1 plant which was homozygous for L. chilense introgressions in chromosomes 3, 6 and 7 was crossed to generate a BC3S1 population which was planted in an infested field. A TYLCV-tolerance gene with partial dominance, TY-1, was mapped to chromosome 6; two modifier genes were mapped to chromosomes 3 and 7. Field and whitefly-mediated cage inoculations of nearly-isogenic lines in BC3S3 supported our conclusion that TY-1 is the major TYLCV-tolerance locus.
KW - Gemini-virus
KW - Lycopersicon
KW - RFLP-assisted selection
KW - Tolerance
KW - Tomato yellow leaf curl virus
UR - http://www.scopus.com/inward/record.url?scp=0028021129&partnerID=8YFLogxK
U2 - 10.1007/BF00225889
DO - 10.1007/BF00225889
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AN - SCOPUS:0028021129
SN - 0040-5752
VL - 88
SP - 141
EP - 146
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
IS - 2
ER -
