TY - JOUR
T1 - Mapping of AvaI and XmaI cleavage sites in bacteriophage DNA including a new technique of DNA digestion in agarose gels
AU - Rosenvold, Eric C.
AU - Honigman, Alik
PY - 1977/12
Y1 - 1977/12
N2 - The eight AvaI cleavage sites in bacteriophage λ DNA have been mapped by analyzing the digestion patterns of various λ deletion-substitution mutants. The size of each DNA fragment has been determined by its mobility in agarose gels and by electron microscopy. The three XmaI cleavage sites were determined by a two dimensional procedure including DNA cleavage in the agarose gel. The fact that the XmaI cleavage site sequence is a subset of the AvaI cleavage site sequences (Hughes and Murray, unpublished; Endow and Roberts, 1979) allowed us to infer the exact DNA sequence at three of the AvaI sites.
AB - The eight AvaI cleavage sites in bacteriophage λ DNA have been mapped by analyzing the digestion patterns of various λ deletion-substitution mutants. The size of each DNA fragment has been determined by its mobility in agarose gels and by electron microscopy. The three XmaI cleavage sites were determined by a two dimensional procedure including DNA cleavage in the agarose gel. The fact that the XmaI cleavage site sequence is a subset of the AvaI cleavage site sequences (Hughes and Murray, unpublished; Endow and Roberts, 1979) allowed us to infer the exact DNA sequence at three of the AvaI sites.
KW - E. coli phage
KW - electron microscopy of DNA fragments
KW - restriction endonucleases
KW - two-dimensional electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=0017564807&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(77)90006-3
DO - 10.1016/0378-1119(77)90006-3
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AN - SCOPUS:0017564807
SN - 0378-1119
VL - 2
SP - 273
EP - 288
JO - Gene
JF - Gene
IS - 5-6
ER -