Abstract
The eight AvaI cleavage sites in bacteriophage λ DNA have been mapped by analyzing the digestion patterns of various λ deletion-substitution mutants. The size of each DNA fragment has been determined by its mobility in agarose gels and by electron microscopy. The three XmaI cleavage sites were determined by a two dimensional procedure including DNA cleavage in the agarose gel. The fact that the XmaI cleavage site sequence is a subset of the AvaI cleavage site sequences (Hughes and Murray, unpublished; Endow and Roberts, 1979) allowed us to infer the exact DNA sequence at three of the AvaI sites.
| Original language | English |
|---|---|
| Pages (from-to) | 273-288 |
| Number of pages | 16 |
| Journal | Gene |
| Volume | 2 |
| Issue number | 5-6 |
| DOIs | |
| State | Published - Dec 1977 |
| Externally published | Yes |
Keywords
- E. coli phage
- electron microscopy of DNA fragments
- restriction endonucleases
- two-dimensional electrophoresis
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