Massively parallel single-nucleus RNA-seq with DroNc-seq

Naomi Habib; Inbal Avraham-Davidi; Anindita Basu; Tyler Burks; Karthik Shekhar; Matan Hofree; Sourav R. Choudhury; François Aguet; Ellen Gelfand; Kristin Ardlie; David A. Weitz; Orit Rozenblatt-Rosen; Feng Zhang; Aviv Regev

doi:10.1038/nmeth.4407

Massively parallel single-nucleus RNA-seq with DroNc-seq

Naomi Habib
, Inbal Avraham-Davidi
, Anindita Basu
, Tyler Burks
, Karthik Shekhar
, Matan Hofree
, Sourav R. Choudhury
, François Aguet
, Ellen Gelfand
, Kristin Ardlie
, David A. Weitz
, Orit Rozenblatt-Rosen
, Feng Zhang
, Aviv Regev^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

786 Scopus citations

Abstract

Single-nucleus RNA sequencing (sNuc-seq) profiles RNA from tissues that are preserved or cannot be dissociated, but it does not provide high throughput. Here, we develop DroNc-seq: massively parallel sNuc-seq with droplet technology. We profile 39,111 nuclei from mouse and human archived brain samples to demonstrate sensitive, efficient, and unbiased classification of cell types, paving the way for systematic charting of cell atlases.

Original language	English
Pages (from-to)	955-958
Number of pages	4
Journal	Nature Methods
Volume	14
Issue number	10
DOIs	https://doi.org/10.1038/nmeth.4407
State	Published - 1 Oct 2017
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2017 Nature America, Inc., part of Springer Nature. All rights reserved.

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10.1038/nmeth.4407

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abstract = "Single-nucleus RNA sequencing (sNuc-seq) profiles RNA from tissues that are preserved or cannot be dissociated, but it does not provide high throughput. Here, we develop DroNc-seq: massively parallel sNuc-seq with droplet technology. We profile 39,111 nuclei from mouse and human archived brain samples to demonstrate sensitive, efficient, and unbiased classification of cell types, paving the way for systematic charting of cell atlases.",

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AU - Avraham-Davidi, Inbal

AU - Basu, Anindita

AU - Burks, Tyler

AU - Shekhar, Karthik

AU - Hofree, Matan

AU - Choudhury, Sourav R.

AU - Aguet, François

AU - Gelfand, Ellen

AU - Ardlie, Kristin

AU - Weitz, David A.

AU - Rozenblatt-Rosen, Orit

AU - Zhang, Feng

AU - Regev, Aviv

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Massively parallel single-nucleus RNA-seq with DroNc-seq

Abstract

Bibliographical note

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