TY - JOUR
T1 - Mast cells and fibroblasts
T2 - two interacting cells
AU - Rubinchik, Evelina
AU - Levi-Schaffer, Francesca
PY - 1994/9
Y1 - 1994/9
N2 - Mast cell (MC) fibroblast interactions may have a role in health and disease. We analyzed the relationships between these cells by utilizing our in vitro model in which mucosal (MMC) and connective tissue (CTMC) type MC were cocultured long-term with different fibroblasts. Mouse 3T3 fibroblasts were used to provide a normal iicroenvironment for MC, while fibroblasts derived from mouse with chronic graft-versushost disease (cGVHD) provided a fibrotic one. We found that both 3T3 and cGVHD fibroblasts maintain CTMC viability, phenotype and functional activity. When MMC were cocultured with 3T3 or cGVHD fibroblasts, they changed their phenotype towards that of CTMC. On the other hand, MCs were found to affect fibroblast properties. Coculture of MMC on 3T3 monolayers was shown to increase Forsmann antigen production and collagen synthesis and stimulate fibroblast proliferation. Resting CTMC or CTMC activated by anaphylactic stimuli induced 3T3 and cGVHD fibroblasts to proliferate more. In addition, CTMC activation increased collagen production by 3T3 fibroblasts. In conclusion, fibroblasts were found to regulate MC survival and differetion, whereas MCs were shown to affect the biochemical properties of fibroblasts, which can lead to fibrosis.
AB - Mast cell (MC) fibroblast interactions may have a role in health and disease. We analyzed the relationships between these cells by utilizing our in vitro model in which mucosal (MMC) and connective tissue (CTMC) type MC were cocultured long-term with different fibroblasts. Mouse 3T3 fibroblasts were used to provide a normal iicroenvironment for MC, while fibroblasts derived from mouse with chronic graft-versushost disease (cGVHD) provided a fibrotic one. We found that both 3T3 and cGVHD fibroblasts maintain CTMC viability, phenotype and functional activity. When MMC were cocultured with 3T3 or cGVHD fibroblasts, they changed their phenotype towards that of CTMC. On the other hand, MCs were found to affect fibroblast properties. Coculture of MMC on 3T3 monolayers was shown to increase Forsmann antigen production and collagen synthesis and stimulate fibroblast proliferation. Resting CTMC or CTMC activated by anaphylactic stimuli induced 3T3 and cGVHD fibroblasts to proliferate more. In addition, CTMC activation increased collagen production by 3T3 fibroblasts. In conclusion, fibroblasts were found to regulate MC survival and differetion, whereas MCs were shown to affect the biochemical properties of fibroblasts, which can lead to fibrosis.
KW - Fibroblasts
KW - Mast cells
UR - http://www.scopus.com/inward/record.url?scp=0028095775&partnerID=8YFLogxK
U2 - 10.1007/BF02592443
DO - 10.1007/BF02592443
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C2 - 7819593
AN - SCOPUS:0028095775
SN - 0940-5437
VL - 24
SP - 139
EP - 142
JO - International Journal of Clinical and Laboratory Research
JF - International Journal of Clinical and Laboratory Research
IS - 3
ER -