Mast cells contribute to the resolution of allergic inflammation by releasing resolvin D1

Pier Giorgio Puzzovio, Hadas Pahima, Tresa George, David Mankuta, Ron Eliashar, Ekaterini Tiligada, Bruce D. Levy, Francesca Levi-Schaffer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Background: Mast cells are initiators and main effectors of allergic inflammation, together with eosinophils, with whom they can interact in a physical and soluble cross-talk with marked pro-inflammatory features, the Allergic Effector Unit. The pro-resolution role of mast cells, alone or in co-culture with eosinophils, has not been characterized yet. Objectives: We aimed to investigate select pro-resolution pathways in mast cells in vitro and in vivo in allergic inflammation. Methods: In vitro, we employed human and murine mast cells and analyzed release of resolvin D1 and expression of 15-lipoxygenase after IgE-mediated activation. We performed co-culture of IgE-activated mast cells with peripheral blood eosinophils and investigated 15-lipoxygenase expression and Resolvin D1 release. In vivo, we performed Ovalbumin/Alum and Ovalbumin/S. aureus enterotoxin B allergic peritonitis model in Wild Type mice following a MC “overshoot” protocol. Results: We found that IgE-activated mast cells release significant amounts of resolvin D1 30 min after activation, while 15-lipoxygenase expression remained unchanged. Resolvin D1 release was found to be decreased in IgE-activated mast cells co-cultured with peripheral blood eosinophils for 30 min In vivo, mast cell-overshoot mice exhibited a trend of reduced inflammation, together with increased peritoneal resolvin D1 release. Conclusions: Mast cells can actively contribute to resolution of allergic inflammation by releasing resolvin D1.

Original languageAmerican English
Article number106691
JournalPharmacological Research
StatePublished - Mar 2023

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  • Allergic inflammation
  • Allergic peritonitis
  • Eosinophils
  • Mast cells
  • Resolution
  • Resolvin D1


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