TY - JOUR
T1 - Measurement of green fluorescence protein in plants
T2 - Quantification, correlation to expression, rapid screening and differential gene expression
AU - Blumenthal, A.
AU - Kuznetzova, L.
AU - Edelbaum, O.
AU - Raskin, V.
AU - Levy, M.
AU - Sela, I.
PY - 1999/3/2
Y1 - 1999/3/2
N2 - A set-up for detection of green fluorescence protein (GFP), enabling rapid screening of transgenic tobacco is described. Fluorescence levels were proportional to expression levels, and expressing plants were visually selected from a transgenic population. Quantification of GFP expression indicated that the 35S CaMV promoter drives expression of this reporter gene to different levels in different tissues, corroborating previous similar observation with GUS, indicating that constitutive expression varies quantitatively in different tissues.
AB - A set-up for detection of green fluorescence protein (GFP), enabling rapid screening of transgenic tobacco is described. Fluorescence levels were proportional to expression levels, and expressing plants were visually selected from a transgenic population. Quantification of GFP expression indicated that the 35S CaMV promoter drives expression of this reporter gene to different levels in different tissues, corroborating previous similar observation with GUS, indicating that constitutive expression varies quantitatively in different tissues.
KW - Gene expression monitoring and quantification
KW - Green fluorescence protein
KW - Plants
UR - http://www.scopus.com/inward/record.url?scp=0033514890&partnerID=8YFLogxK
U2 - 10.1016/S0168-9452(98)00249-0
DO - 10.1016/S0168-9452(98)00249-0
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0033514890
SN - 0168-9452
VL - 142
SP - 93
EP - 99
JO - Plant Science
JF - Plant Science
IS - 1
ER -