Measurement of green fluorescence protein in plants: Quantification, correlation to expression, rapid screening and differential gene expression

A. Blumenthal; L. Kuznetzova; O. Edelbaum; V. Raskin; M. Levy; I. Sela

doi:10.1016/S0168-9452(98)00249-0

Measurement of green fluorescence protein in plants: Quantification, correlation to expression, rapid screening and differential gene expression

A. Blumenthal^*
, L. Kuznetzova
, O. Edelbaum
, V. Raskin
, M. Levy
, I. Sela

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

A set-up for detection of green fluorescence protein (GFP), enabling rapid screening of transgenic tobacco is described. Fluorescence levels were proportional to expression levels, and expressing plants were visually selected from a transgenic population. Quantification of GFP expression indicated that the 35S CaMV promoter drives expression of this reporter gene to different levels in different tissues, corroborating previous similar observation with GUS, indicating that constitutive expression varies quantitatively in different tissues.

Original language	English
Pages (from-to)	93-99
Number of pages	7
Journal	Plant Science
Volume	142
Issue number	1
DOIs	https://doi.org/10.1016/S0168-9452(98)00249-0
State	Published - 2 Mar 1999

Keywords

Gene expression monitoring and quantification
Green fluorescence protein
Plants

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10.1016/S0168-9452(98)00249-0

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title = "Measurement of green fluorescence protein in plants: Quantification, correlation to expression, rapid screening and differential gene expression",

abstract = "A set-up for detection of green fluorescence protein (GFP), enabling rapid screening of transgenic tobacco is described. Fluorescence levels were proportional to expression levels, and expressing plants were visually selected from a transgenic population. Quantification of GFP expression indicated that the 35S CaMV promoter drives expression of this reporter gene to different levels in different tissues, corroborating previous similar observation with GUS, indicating that constitutive expression varies quantitatively in different tissues.",

keywords = "Gene expression monitoring and quantification, Green fluorescence protein, Plants",

author = "A. Blumenthal and L. Kuznetzova and O. Edelbaum and V. Raskin and M. Levy and I. Sela",

year = "1999",

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T1 - Measurement of green fluorescence protein in plants

T2 - Quantification, correlation to expression, rapid screening and differential gene expression

AU - Blumenthal, A.

AU - Kuznetzova, L.

AU - Edelbaum, O.

AU - Raskin, V.

AU - Levy, M.

AU - Sela, I.

PY - 1999/3/2

Y1 - 1999/3/2

N2 - A set-up for detection of green fluorescence protein (GFP), enabling rapid screening of transgenic tobacco is described. Fluorescence levels were proportional to expression levels, and expressing plants were visually selected from a transgenic population. Quantification of GFP expression indicated that the 35S CaMV promoter drives expression of this reporter gene to different levels in different tissues, corroborating previous similar observation with GUS, indicating that constitutive expression varies quantitatively in different tissues.

AB - A set-up for detection of green fluorescence protein (GFP), enabling rapid screening of transgenic tobacco is described. Fluorescence levels were proportional to expression levels, and expressing plants were visually selected from a transgenic population. Quantification of GFP expression indicated that the 35S CaMV promoter drives expression of this reporter gene to different levels in different tissues, corroborating previous similar observation with GUS, indicating that constitutive expression varies quantitatively in different tissues.

KW - Gene expression monitoring and quantification

KW - Green fluorescence protein

KW - Plants

UR - https://www.scopus.com/pages/publications/0033514890

U2 - 10.1016/S0168-9452(98)00249-0

DO - 10.1016/S0168-9452(98)00249-0

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AN - SCOPUS:0033514890

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JF - Plant Science

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ER -

Measurement of green fluorescence protein in plants: Quantification, correlation to expression, rapid screening and differential gene expression

Abstract

Keywords

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