Measurement of lipid forces by X-ray diffraction and osmotic stress

Horia I. Petrache, Daniel Harries, V. Adrian Parsegian

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

14 Scopus citations


Lipid suspensions in aqueous solutions most often form multilamellar vesicles of uniformly spaced bilayers. Interlamellar spacing is determined by the balance of attractive van der Waals (charge fluctuation) and repulsive forces. This balance of forces, as well as membrane elasticity, can be probed by applied osmotic stress. We describe how osmotic stress can be imposed on multilamellar lipid samples to study lipid interactions.

Original languageAmerican English
Title of host publicationMethods in Membrane Lipids
PublisherHumana Press
Number of pages15
ISBN (Print)1597455199, 9781597455190
StatePublished - 30 Aug 2007

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Bending rigidity
  • Cholesterol hydration
  • Membrane fluctuations
  • Osmotic pressure
  • Spontaneous curvature


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