TY - JOUR
T1 - Mechanisms involved in the weak alloimmunogenicity of Thy-1 on mouse brain
AU - Lobel, Steven
AU - Rabinowitz, Ruth
AU - Schlesinger, Michael
PY - 1979/10
Y1 - 1979/10
N2 - Immunization of mice with allogeneic brain homogenates fails to elicit an appreciable response to the Thy-1 antigen. The aim of the present study was to determine the mechanism of the low immunogenicity of allogeneic brain.Immunization of C3H mice with freshly prepared mixtures of AKR/J thymus cells with either AKR/J or C3H brain homogenates elicited a primary response to the Thy-1.1 antigen as effectively as immunization with AKR/J thymus cells. When the mixtures were incubated overnight prior to injection, only AKR/J brain homogenate but not C3H brain homogenate abrogated the capacity of AKR/J thymus cells to elicit an anti-Thy-1.1 response. Suppressor T cells did not seem to be responsible for the inability of brain to elicit an anti-Thy-1 response, as mice that received cyclophosphamide 2 days prior to injection of brain did not produce Thy-1 antibodies. Antigenic competition also did not seem to be the cause for the weak immunogenicity of Thy-1 on brain, as mixtures of thymus and brain were capable of eliciting a primary response.When spleen cells from mice hyperimmunized against Thy-1 were transferred to normal syngeneic mice, subsequent immunization with brain homogenate was capable of eliciting Thy-1 antibodies. These results indicate that Thy-1 on brain may resemble a hapten, in being incapable of eliciting a primary immune response, but behaves like a complete antigen in boosting a secondary response.Thy-1 antibodies can be readily elicited by immunization of allogeneic hosts with lymphoid cells . Rabbits or rats immunized with mouse brain homogenates also form Thy-1 antibodies . In allogeneic hosts, however, brain homogenates are very deficient in their capacity to elicit the production of Thy-1 antibodies. The discrepency between the high antigenicity of brain as evidenced by its capacity of absorbing alloantibodies and its weak immunogenic activity could be attributed to a number of mechanisms discussed previously (7). It has recently been suggested that the immune response to Thy-1 present on thymus cells is regulated by the concomitant presence of other antigens that may act as either intermolecular helpers or competitive inhibitors . The aim of this study was to delineate the factors involved in the weak alloantigenicity of Thy-1 in the brain.
AB - Immunization of mice with allogeneic brain homogenates fails to elicit an appreciable response to the Thy-1 antigen. The aim of the present study was to determine the mechanism of the low immunogenicity of allogeneic brain.Immunization of C3H mice with freshly prepared mixtures of AKR/J thymus cells with either AKR/J or C3H brain homogenates elicited a primary response to the Thy-1.1 antigen as effectively as immunization with AKR/J thymus cells. When the mixtures were incubated overnight prior to injection, only AKR/J brain homogenate but not C3H brain homogenate abrogated the capacity of AKR/J thymus cells to elicit an anti-Thy-1.1 response. Suppressor T cells did not seem to be responsible for the inability of brain to elicit an anti-Thy-1 response, as mice that received cyclophosphamide 2 days prior to injection of brain did not produce Thy-1 antibodies. Antigenic competition also did not seem to be the cause for the weak immunogenicity of Thy-1 on brain, as mixtures of thymus and brain were capable of eliciting a primary response.When spleen cells from mice hyperimmunized against Thy-1 were transferred to normal syngeneic mice, subsequent immunization with brain homogenate was capable of eliciting Thy-1 antibodies. These results indicate that Thy-1 on brain may resemble a hapten, in being incapable of eliciting a primary immune response, but behaves like a complete antigen in boosting a secondary response.Thy-1 antibodies can be readily elicited by immunization of allogeneic hosts with lymphoid cells . Rabbits or rats immunized with mouse brain homogenates also form Thy-1 antibodies . In allogeneic hosts, however, brain homogenates are very deficient in their capacity to elicit the production of Thy-1 antibodies. The discrepency between the high antigenicity of brain as evidenced by its capacity of absorbing alloantibodies and its weak immunogenic activity could be attributed to a number of mechanisms discussed previously (7). It has recently been suggested that the immune response to Thy-1 present on thymus cells is regulated by the concomitant presence of other antigens that may act as either intermolecular helpers or competitive inhibitors . The aim of this study was to delineate the factors involved in the weak alloantigenicity of Thy-1 in the brain.
UR - http://www.scopus.com/inward/record.url?scp=0018656222&partnerID=8YFLogxK
U2 - 10.1097/00007890-197910000-00013
DO - 10.1097/00007890-197910000-00013
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C2 - 505543
AN - SCOPUS:0018656222
SN - 0041-1337
VL - 28
SP - 329
EP - 332
JO - Transplantation
JF - Transplantation
IS - 4
ER -