Abstract
It was shown that perfusion of the isolated heart of rat with solution containing the CB1- and CB2-receptor agonist HU-210 at concentrations of 0.1 or 1.0 μM/L for a duration of 10 min at 20 min before global ischemia (45 min) and reperfusion (30 min) promotes a twofold decrease in creatine kinase levels in coronary effluent. It was established that KATP channel blockade by glibenclamide (1 μM/L) or inhibition of protein kinase C (2 μM/L) by chelerythrine abolishes the cardioprotective effect of HU-210. The inhibitor of NO synthase L-NAME (1 μM/L) had no effect on the anti-necrotic effect of HU-210. Thus, the intracellular signaling mechanism of the cardioprotective effect of the CB-agonist HU-210 involves the activation of KATP channels and protein kinase C without the participation of NO-synthase.
Original language | English |
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Pages (from-to) | 15-18 |
Number of pages | 4 |
Journal | Eksperimental'naya i Klinicheskaya Farmakologiya |
Volume | 75 |
Issue number | 12 |
State | Published - 2012 |
Keywords
- Cannabinoid receptors
- Heart
- Ischemia
- Reperfusion