Membrane localization is critical for activation of the PICK1 BAR domain

Kenneth L. Madsen, Jacob Eriksen, Laura Milan-Lobo, Daniel S. Han, Masha Y. Niv, Ina Ammendrup-Johnsen, Ulla Henriksen, Vikram K. Bhatia, Dimitrios Stamou, Harald H. Sitte, Harvey T. McMahon, Harel Weinstein, Ulrik Gether*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


The PSD-95/Discs-large/ZO-1 homology (PDZ) domain protein, protein interacting with C kinase 1 (PICK1) contains a C-terminal Bin/ amphiphysin/Rvs (BAR) domain mediating recognition of curved membranes; however, the molecular mechanisms controlling the activity of this domain are poorly understood. In agreement with negative regulation of the BAR domain by the N-terminal PDZ domain, PICK1 distributed evenly in the cytoplasm, whereas truncation of the PDZ domain caused BAR domain-dependent redistribution to clusters colocalizing with markers of recycling endosomal compartments. A similar clustering was observed both upon truncation of a short putative α-helical segment in the linker between the PDZ and the BAR domains and upon coexpression of PICK1 with a transmembrane PDZ ligand, including the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor GluR2 subunit, the GluR2 C-terminus transferred to the single transmembrane protein Tac or the dopamine transporter C-terminus transferred to Tac. In contrast, transfer of the GluR2 C-terminus to cyan fluorescent protein, a cytosolic protein, did not elicit BAR domain-dependent clustering. Instead, localizing PICK1 to the membrane by introducing an N-terminal myristoylation site produced BAR domain-dependent, but ligand-independent, PICK1 clustering. The data support that in the absence of PDZ ligand, the PICK1 BAR domain is inhibited through a PDZ domain-dependent and linker-dependent mechanism. Moreover, they suggest that unmasking of the BAR domain's membrane-binding capacity is not a consequence of ligand binding to the PDZ domain per se but results from, and coincides with, recruitment of PICK1 to a membrane compartment.

Original languageAmerican English
Pages (from-to)1327-1343
Number of pages17
Issue number8
StatePublished - Aug 2008
Externally publishedYes


  • BAR domains
  • PDZ domains
  • Protein-lipid interactions
  • Receptors
  • Transporters


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