TY - JOUR
T1 - Membrane water permeability and aquaporin expression increase during growth of maize suspension cultured cells
AU - Moshelion, Menachem
AU - Hachez, Charles
AU - Ye, Qing
AU - Cavez, Damien
AU - Bajji, Mohammed
AU - Jung, Rudolf
AU - Chaumont, François
PY - 2009/10
Y1 - 2009/10
N2 - Aquaporins (AQPs) are water channels that allow cells to rapidly alter their membrane water permeability. A convenient model for studying AQP expression and activity regulation is Black Mexican Sweet (BMS) maize cultured cells. In an attempt to correlate membrane osmotic water permeability coefficient (Pf) with AQP gene expression, we first examined the expression pattern of 33 AQP genes using macro-array hybridization. We detected the expression of 18 different isoforms representing the four AQP subfamilies, i.e. eight plasma membrane (PIP), five tonoplast (TIP), three small basic (SIP) and two NOD26-like (NIP) AQPs. While the expression of most of these genes was constant throughout all growth phases, mRNA levels of ZmPIP1;3, ZmPIP2;1, ZmPIP2;2, ZmPIP2;4 and ZmPIP2;6 increased significantly during the logarithmic growth phase and the beginning of the stationary phase. The use of specific anti-ZmPIP antisera showed that the protein expression pattern correlated well with mRNA levels. Cell pressure probe and protoplast swelling measurements were then performed to determine the Pf. Interestingly, we found that the Pf were significantly increased at the end of the logarithmic growth phase and during the steady-state phase compared to the lag phase, demonstrating a positive correlation between AQP abundance in the plasma membrane and the cell Pf.
AB - Aquaporins (AQPs) are water channels that allow cells to rapidly alter their membrane water permeability. A convenient model for studying AQP expression and activity regulation is Black Mexican Sweet (BMS) maize cultured cells. In an attempt to correlate membrane osmotic water permeability coefficient (Pf) with AQP gene expression, we first examined the expression pattern of 33 AQP genes using macro-array hybridization. We detected the expression of 18 different isoforms representing the four AQP subfamilies, i.e. eight plasma membrane (PIP), five tonoplast (TIP), three small basic (SIP) and two NOD26-like (NIP) AQPs. While the expression of most of these genes was constant throughout all growth phases, mRNA levels of ZmPIP1;3, ZmPIP2;1, ZmPIP2;2, ZmPIP2;4 and ZmPIP2;6 increased significantly during the logarithmic growth phase and the beginning of the stationary phase. The use of specific anti-ZmPIP antisera showed that the protein expression pattern correlated well with mRNA levels. Cell pressure probe and protoplast swelling measurements were then performed to determine the Pf. Interestingly, we found that the Pf were significantly increased at the end of the logarithmic growth phase and during the steady-state phase compared to the lag phase, demonstrating a positive correlation between AQP abundance in the plasma membrane and the cell Pf.
KW - Aquaporins
KW - Cell pressure probe
KW - Expression
KW - Hydraulic conductivity
KW - Osmotic water permeability coefficient
KW - Plasma membrane
KW - Protoplasts
KW - Suspension cells
KW - Water
UR - http://www.scopus.com/inward/record.url?scp=69949184326&partnerID=8YFLogxK
U2 - 10.1111/j.1365-3040.2009.02001.x
DO - 10.1111/j.1365-3040.2009.02001.x
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C2 - 19453479
AN - SCOPUS:69949184326
SN - 0140-7791
VL - 32
SP - 1334
EP - 1345
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
IS - 10
ER -