Metabolic Engineering Strategy Enables a Hundred-Fold Increase in Viniferin Levels in Vitis vinifera cv. Gamay Red Cell Culture

Ru Wang, Sangram Keshari Lenka, Varun Kumar, Kelem Gashu, Noga Sikron-Persi, Irena Dynkin, David Weiss, Avichai Perl, Aaron Fait, Michal Oren-Shamir*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Stilbenes are phytoalexins with health-promoting benefits for humans. Here, we boost stilbenes' production, and in particular the resveratrol dehydrodimer viniferin, with significant pharmacological properties, by overexpressing stilbene synthase (STS) under unlimited phenylalanine (Phe) supply. Vitis vinifera cell cultures were co-transformed with a feedback-insensitive E. coli DAHP synthase (AroG*) and STS genes, under constitutive promoters. All transgenic lines had increased levels of Phe and stilbenes (74-fold higher viniferin reaching 0.74 mg/g DW). External Phe feeding of AroG∗ + STS lines caused a synergistic effect on resveratrol and viniferin accumulation, achieving a 26-fold (1.33 mg/g DW) increase in resveratrol and a 620-fold increase (6.2 mg/g DW) in viniferin, which to date is the highest viniferin accumulation reported in plant cultures. We suggest that this strategy of combining higher Phe availability and STS expression generates grape cell cultures as potential factories for sustainable production of stilbenes with a minor effect on the levels of flavonoids.

Original languageAmerican English
Pages (from-to)3124-3133
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume69
Issue number10
DOIs
StatePublished - 17 Mar 2021

Bibliographical note

Publisher Copyright:
© 2021 American Chemical Society.

Keywords

  • Vitis vinifera
  • grape cell culture
  • phenylalanine
  • resveratrol
  • stilbenes
  • viniferin

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