Metabolic imaging with hyperpolarized [1-¹³C] pyruvate in patient-derived preclinical mouse models of breast cancer

¹³C nuclear spin hyperpolarization can increase the sensitivity of detection in an MRI experiment by more than 10,000-fold. ¹³C magnetic resonance spectroscopic imaging (MRSI) of hyperpolarized ¹³C label exchange between injected [1-¹³C]pyruvate and the endogenous tumor lactate pool can be used clinically to assess tumor grade and response to treatment. We describe here an experimental protocol for using this technique in patient-derived and established cell line xenograft models of breast cancer in the mouse. For complete details on the use and execution of this protocol, please refer to Ros et al. (2020).