Metabolically controlled hemolysis of chicken erythrocytes

Amos Frish*, Yair Gazitt, Abraham Loyter

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Commercial preparations of phospholipase C (Clostridium perfringens) contain a hemolysin which hemolyses red blood cells in the absence of bivalent cations and a phospholipase C whose activity is dependent on bivalent metals. The hemolysin readily lysed fresh chicken erythrocytes but failed to act on ATP-depleted cells. Heating of the commercial preparation of phospholipase C caused inactivation of the hemolytic activity while the hydrolytic activity remained relatively intact. A heated preparation failed to affect fresh erythrocytes but readily hemolysed ATP-depleted cells. Hemolysis of ATP-depleted cells took place only in the presence of bivalent cations such as Ca2+ or Mn2+. ATP-depleted cells became sensitive to the hemolysin and resistant to the heated preparation again after restoration of the internal pool of ATP. It is suggested that ATP dependent changes of the erythrocyte membrane are responsible for these changes in sensitivity.

Original languageEnglish
Pages (from-to)690-700
Number of pages11
JournalBiochimica et Biophysica Acta - Biomembranes
Volume291
Issue number3
DOIs
StatePublished - 16 Feb 1973

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