TY - JOUR
T1 - Metagenomic mining for thermostable esterolytic enzymes uncovers a new family of bacterial esterases
AU - Zarafeta, Dimitra
AU - Moschidi, Danai
AU - Ladoukakis, Efthymios
AU - Gavrilov, Sergey
AU - Chrysina, Evangelia D.
AU - Chatziioannou, Aristotelis
AU - Kublanov, Ilya
AU - Skretas, Georgios
AU - Kolisis, Fragiskos N.
N1 - Publisher Copyright:
© The Author(s) 2016.
PY - 2016/12/19
Y1 - 2016/12/19
N2 - Biocatalysts exerting activity against ester bonds have a broad range of applications in modern biotechnology. Here, we have identified a new esterolytic enzyme by screening a metagenomic sample collected from a hot spring in Kamchatka, Russia. Biochemical characterization of the new esterase, termed EstDZ2, revealed that it is highly active against medium chain fatty acid esters at temperatures between 25 and 60 °C and at pH values 7-8. The new enzyme is moderately thermostable with a half-life of more than six hours at 60 °C, but exhibits exquisite stability against high concentrations of organic solvents. Phylogenetic analysis indicated that EstDZ2 is likely an Acetothermia enzyme that belongs to a new family of bacterial esterases, for which we propose the index XV. One distinctive feature of this new family, is the presence of a conserved GHSAG catalytic motif. Multiple sequence alignment, coupled with computational modelling of the three-dimensional structure of EstDZ2, revealed that the enzyme lacks the largest part of the "cap" domain, whose extended structure is characteristic for the closely related Family IV esterases. Thus, EstDZ2 appears to be distinct from known related esterolytic enzymes, both in terms of sequence characteristics, as well as in terms of three-dimensional structure.
AB - Biocatalysts exerting activity against ester bonds have a broad range of applications in modern biotechnology. Here, we have identified a new esterolytic enzyme by screening a metagenomic sample collected from a hot spring in Kamchatka, Russia. Biochemical characterization of the new esterase, termed EstDZ2, revealed that it is highly active against medium chain fatty acid esters at temperatures between 25 and 60 °C and at pH values 7-8. The new enzyme is moderately thermostable with a half-life of more than six hours at 60 °C, but exhibits exquisite stability against high concentrations of organic solvents. Phylogenetic analysis indicated that EstDZ2 is likely an Acetothermia enzyme that belongs to a new family of bacterial esterases, for which we propose the index XV. One distinctive feature of this new family, is the presence of a conserved GHSAG catalytic motif. Multiple sequence alignment, coupled with computational modelling of the three-dimensional structure of EstDZ2, revealed that the enzyme lacks the largest part of the "cap" domain, whose extended structure is characteristic for the closely related Family IV esterases. Thus, EstDZ2 appears to be distinct from known related esterolytic enzymes, both in terms of sequence characteristics, as well as in terms of three-dimensional structure.
UR - http://www.scopus.com/inward/record.url?scp=85006390460&partnerID=8YFLogxK
U2 - 10.1038/srep38886
DO - 10.1038/srep38886
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C2 - 27991516
AN - SCOPUS:85006390460
SN - 2045-2322
VL - 6
JO - Scientific Reports
JF - Scientific Reports
M1 - 38886
ER -