Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing

Bradley Bernstein; Alon Goren; Chad Nusbaum; Oren Ram; Assaf Rotem; Daniel Tarjan; Jeffrey Xing; Aviv Regev

Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing

Bradley Bernstein (Inventor), Alon Goren (Inventor), Chad Nusbaum (Inventor), Oren Ram (Inventor), Assaf Rotem (Inventor), Daniel Tarjan (Inventor), Jeffrey Xing (Inventor), Aviv Regev (Inventor)

Department of Biological Chemistry

Research output: Patent

Abstract

Disclosed are methods for shearing and tagging chromatin DNA. The disclosed methods include contacting chromatin DNA with at least one transposome, that includes a transposase enzyme. The transposon is made up of a first DNA molecule that includes a first transposase recognition site and a second DNA molecule that includes a second transposase recognition site, wherein the transposase integrates the first and second DNA molecules into chromatin DNA. The first and second DNA molecules of the transposon can be disconnected, such that upon integration of the transposon the chromatin bound DNA is sheared and tagged with the first and second DNA molecules, for example to prepare a library of sheared and tagged chromatin DNA fragments.

Original language	American English
Patent number	US2016208323
IPC	G01N 33/ 541 A I
Priority date	19/06/14
State	Published - 21 Jul 2016

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title = "Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing",

abstract = "Disclosed are methods for shearing and tagging chromatin DNA. The disclosed methods include contacting chromatin DNA with at least one transposome, that includes a transposase enzyme. The transposon is made up of a first DNA molecule that includes a first transposase recognition site and a second DNA molecule that includes a second transposase recognition site, wherein the transposase integrates the first and second DNA molecules into chromatin DNA. The first and second DNA molecules of the transposon can be disconnected, such that upon integration of the transposon the chromatin bound DNA is sheared and tagged with the first and second DNA molecules, for example to prepare a library of sheared and tagged chromatin DNA fragments.",

author = "Bradley Bernstein and Alon Goren and Chad Nusbaum and Oren Ram and Assaf Rotem and Daniel Tarjan and Jeffrey Xing and Aviv Regev",

year = "2016",

month = jul,

day = "21",

language = "American English",

type = "Patent",

note = "US2016208323; G01N 33/ 541 A I",

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TY - PAT

T1 - Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing

AU - Bernstein, Bradley

AU - Goren, Alon

AU - Nusbaum, Chad

AU - Ram, Oren

AU - Rotem, Assaf

AU - Tarjan, Daniel

AU - Xing, Jeffrey

AU - Regev, Aviv

PY - 2016/7/21

Y1 - 2016/7/21

N2 - Disclosed are methods for shearing and tagging chromatin DNA. The disclosed methods include contacting chromatin DNA with at least one transposome, that includes a transposase enzyme. The transposon is made up of a first DNA molecule that includes a first transposase recognition site and a second DNA molecule that includes a second transposase recognition site, wherein the transposase integrates the first and second DNA molecules into chromatin DNA. The first and second DNA molecules of the transposon can be disconnected, such that upon integration of the transposon the chromatin bound DNA is sheared and tagged with the first and second DNA molecules, for example to prepare a library of sheared and tagged chromatin DNA fragments.

AB - Disclosed are methods for shearing and tagging chromatin DNA. The disclosed methods include contacting chromatin DNA with at least one transposome, that includes a transposase enzyme. The transposon is made up of a first DNA molecule that includes a first transposase recognition site and a second DNA molecule that includes a second transposase recognition site, wherein the transposase integrates the first and second DNA molecules into chromatin DNA. The first and second DNA molecules of the transposon can be disconnected, such that upon integration of the transposon the chromatin bound DNA is sheared and tagged with the first and second DNA molecules, for example to prepare a library of sheared and tagged chromatin DNA fragments.

M3 - Patent

M1 - US2016208323

ER -

Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing

Abstract

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