Methods for studying RNA localization in bacteria

Shanmugapriya Kannaiah; Orna Amster-Choder

doi:10.1016/j.ymeth.2015.12.010

Methods for studying RNA localization in bacteria

Shanmugapriya Kannaiah, Orna Amster-Choder^*

^*Corresponding author for this work

Department of Microbiology and Molecular Genetics

Research output: Contribution to journal › Review article › peer-review

14 Scopus citations

Abstract

The subcellular localization of RNA transcripts provides important insights into biological processes. Hence, understanding the mechanisms underlying RNA targeting is a high priority aim of modern cell biology. The advancements in imaging techniques, such as in situ hybridization and live-cell imaging, coupled with the evolution in optical microscopy led to the discovery that bacterial RNAs, despite the lack of nucleus, are specifically localized. Here we describe the methods used to study RNA localization in bacteria and their applications and discuss their advantages and limitations.

Original language	English
Pages (from-to)	99-103
Number of pages	5
Journal	Methods
Volume	98
DOIs	https://doi.org/10.1016/j.ymeth.2015.12.010
State	Published - 1 Apr 2016

Bibliographical note

Funding Information:
We thank members of Orna Amster-Choder’s lab for fruitful discussions. This work was supported by the Israel Science Foundation founded by the Israel Academy of Sciences and Humanities .

Publisher Copyright:
© 2015 Elsevier Inc.

Keywords

Bacterial cell
Fluorescence microscopy
Imaging techniques
Quenching
RNA localization
Super-resolution microscopy

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10.1016/j.ymeth.2015.12.010

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KW - Fluorescence microscopy

KW - Imaging techniques

KW - Quenching

KW - RNA localization

KW - Super-resolution microscopy

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Methods for studying RNA localization in bacteria

Abstract

Bibliographical note

Keywords

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