Methods for studying RNA localization in bacteria

Shanmugapriya Kannaiah, Orna Amster-Choder*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

14 Scopus citations

Abstract

The subcellular localization of RNA transcripts provides important insights into biological processes. Hence, understanding the mechanisms underlying RNA targeting is a high priority aim of modern cell biology. The advancements in imaging techniques, such as in situ hybridization and live-cell imaging, coupled with the evolution in optical microscopy led to the discovery that bacterial RNAs, despite the lack of nucleus, are specifically localized. Here we describe the methods used to study RNA localization in bacteria and their applications and discuss their advantages and limitations.

Original languageAmerican English
Pages (from-to)99-103
Number of pages5
JournalMethods
Volume98
DOIs
StatePublished - 1 Apr 2016

Bibliographical note

Funding Information:
We thank members of Orna Amster-Choder’s lab for fruitful discussions. This work was supported by the Israel Science Foundation founded by the Israel Academy of Sciences and Humanities .

Publisher Copyright:
© 2015 Elsevier Inc.

Keywords

  • Bacterial cell
  • Fluorescence microscopy
  • Imaging techniques
  • Quenching
  • RNA localization
  • Super-resolution microscopy

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