TY - JOUR
T1 - Micro-electroporation of mesenchymal stem cells with alternating electrical current pulses
AU - Ziv, Roee
AU - Steinhardt, Yair
AU - Pelled, Gadi
AU - Gazit, Dan
AU - Rubinsky, Boris
PY - 2009
Y1 - 2009
N2 - Micro-electroporation is an electroporation technology in which the electrical field that induces cell membrane poration is focused onto a single cell contained in a micro-electromechanical structure. Micro-electroporation has many unique attributes including that it facilitates real time control over the process of electroporation at the single cell level. Flow-through micro-electroporation expands on this principle and was developed to facilitate electroporation of a large numbers of cells with control over the electroporation of every single cell. However, our studies show that when electroporation employs conventional direct current (DC) electrical pulses the micro-electroporation system fails, because of electrolysis induced gas bubble formation. We report in this study that when certain alternating currents (AC) electrical pulses are used for micro-electroporation it becomes possible to avoid electrolytic gas bubble formation in a micro-electroporation flow-through system. The effect of AC micro-electroporation on electrolysis was found to depend on the AC frequency used. This concept was tested with mesenchymal stem cells and preliminary results show successful electroporation using this system.
AB - Micro-electroporation is an electroporation technology in which the electrical field that induces cell membrane poration is focused onto a single cell contained in a micro-electromechanical structure. Micro-electroporation has many unique attributes including that it facilitates real time control over the process of electroporation at the single cell level. Flow-through micro-electroporation expands on this principle and was developed to facilitate electroporation of a large numbers of cells with control over the electroporation of every single cell. However, our studies show that when electroporation employs conventional direct current (DC) electrical pulses the micro-electroporation system fails, because of electrolysis induced gas bubble formation. We report in this study that when certain alternating currents (AC) electrical pulses are used for micro-electroporation it becomes possible to avoid electrolytic gas bubble formation in a micro-electroporation flow-through system. The effect of AC micro-electroporation on electrolysis was found to depend on the AC frequency used. This concept was tested with mesenchymal stem cells and preliminary results show successful electroporation using this system.
KW - Cell membrane
KW - Electrolysis
KW - Mesenchymal stem cells
KW - Micro-channel
KW - Micro-electroporation
KW - Transmembrane potential
UR - http://www.scopus.com/inward/record.url?scp=59949102798&partnerID=8YFLogxK
U2 - 10.1007/s10544-008-9213-4
DO - 10.1007/s10544-008-9213-4
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 18815886
AN - SCOPUS:59949102798
SN - 1387-2176
VL - 11
SP - 95
EP - 101
JO - Biomedical Microdevices
JF - Biomedical Microdevices
IS - 1
ER -