Microenvironment of the binding site in the lac carrier protein

S. Schuldiner, R. Weil, D. E. Robertson, H. R. Kaback

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16 Scopus citations

Abstract

Studies with a homologous series of (N dansyl)aminoalkyl 1 thio β D galactopyranosides containing 2 to 6 methylene carbons bridging the galactosyl and dansyl ends of the molecules are described. The compounds were utilized in radioactive and nonradioactive form, and binding of each homologue to membrane vesicles isolated from Escherichia coli ML 308 225 was measured directly by flow dialysis in the presence of D lactate. The results are compared with the D lactate induced fluorescence enhancement observed with each dansylgalactoside and with the ability of N methylpicolinium perchlorate to quench the fluorescence of the bound homologues. The binding affinity of the lac carrier protein for the probes varies directly with the length of the alkyl linkage, and the same number of binding sites is observed with each homologue. In contrast, however, the increase in fluorescence observed upon binding varies dramatically as the alkyl chain is increased in length, with the fluorescence exhibiting maximal values at 2 and 6 methylene carbons and a minimum at 4 methylene carbons. Furthermore, quenching by N methyl picolinium perchlorate exhibits an inverse relationship and maximum quenching is observed with the 4 carbon homologue. Possible reasons for this behavior are discussed.

Original languageEnglish
Pages (from-to)1851-1854
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume74
Issue number5
DOIs
StatePublished - 1977
Externally publishedYes

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