Microfluidic concentric gradient generator design for high-Throughput cell-Based studies

Elishai Ezra Tsur, Michal Zimerman, Idan Maor, Avner Elrich, Yaakov Nahmias*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Gradients of diffusible signaling molecules play important role in various processes, ranging from cell differentiation to toxicological evaluation. Microfluidic technology provides an accurate control of tempospatial conditions. However, current microfluidic platforms are not designed to handle multiple gradients and cell populations simultaneously. Here, we demonstrate a rapidly adaptable microfluidic design able to expose multiple cell populations to an array of chemical gradients. Our design is based on pressure-equilibrated concentric channels and a pressure-dissipating control layer, facilitating the seeding of multiple cell populations in a single device. The design was numerically evaluated and experimentally validated. The device consists of 8 radiating stimuli channels and 12 circular cell culture channels, creating an array of 96 different continuous gradients that can be simultaneously monitored over time.

Original languageAmerican English
Article number21
JournalFrontiers in Bioengineering and Biotechnology
Volume5
Issue numberAPR
DOIs
StatePublished - 12 Apr 2017

Bibliographical note

Publisher Copyright:
© 2017 Ezra Tsur, Zimerman, Maor, Elrich and Nahmias.

Keywords

  • Biomems
  • Computer-aided design
  • High-content studies
  • Numerical modeling
  • Soft lithography

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