TY - JOUR
T1 - Microgravimetric quartz-crystal microbalance analysis of cytochrome c interactions with pyridine and pyridine-nitrospiropyran monolayer electrodes
T2 - Characterization of inter-protein complexes at the functionalized surfaces
AU - Lion-Dagan, Mazzi
AU - Ben-Dov, Iddo
AU - Willner, Itamar
PY - 1997/4/10
Y1 - 1997/4/10
N2 - The association of cytochrome c (Cyt. c) to a pyridine monolayer assembled onto Au electrodes associated with a quartz crystal is followed microgravimetrically using a quartz-crystal microbalance (QCM). The association constant of Cyt. c to the monolayer corresponds to K1 = 8.5 x 103 M-1. The inter-protein binding of cytochrome oxidase (COx) to a pyridine-Cyt. c monolayer assembled on the electrode is similarly characterized by the QCM method. The association constant of COx to Cyt. c is K2 = 1.67 x 106 M-2. A mixed monolayer consisting of pyridine and nitrospiropyran units was assembled on Au electrodes associated with a quartz crystal. The functionalized monolayer was used to photostimulate the association and dissociation of Cyt. c to the monolayer. The system provides a means for the piezoelectric microgravimetric transduction of optical signals recorded by the monolayer-modified surface.
AB - The association of cytochrome c (Cyt. c) to a pyridine monolayer assembled onto Au electrodes associated with a quartz crystal is followed microgravimetrically using a quartz-crystal microbalance (QCM). The association constant of Cyt. c to the monolayer corresponds to K1 = 8.5 x 103 M-1. The inter-protein binding of cytochrome oxidase (COx) to a pyridine-Cyt. c monolayer assembled on the electrode is similarly characterized by the QCM method. The association constant of COx to Cyt. c is K2 = 1.67 x 106 M-2. A mixed monolayer consisting of pyridine and nitrospiropyran units was assembled on Au electrodes associated with a quartz crystal. The functionalized monolayer was used to photostimulate the association and dissociation of Cyt. c to the monolayer. The system provides a means for the piezoelectric microgravimetric transduction of optical signals recorded by the monolayer-modified surface.
KW - Cytochrome c
KW - Cytochrome oxidase
KW - Photoisomerizable monolayer
KW - Protein association
KW - Quartz crystal microbalance
KW - Self-assembled monolayer
UR - http://www.scopus.com/inward/record.url?scp=0031452368&partnerID=8YFLogxK
U2 - 10.1016/S0927-7765(97)00008-8
DO - 10.1016/S0927-7765(97)00008-8
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AN - SCOPUS:0031452368
SN - 0927-7765
VL - 8
SP - 251
EP - 260
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - 4-5
ER -