TY - JOUR
T1 - Microinjected Xenopus oocytes synthesize active human plasminogen activator
AU - Miskin, Ruth
AU - Soreq, Hermona
PY - 1981/7/24
Y1 - 1981/7/24
N2 - Induction of synthesis of the protease plasminogen activator (PA) by hormones, oncogenic viruses and tumor promoters occurs at the transcription level. A novel bioassay for PA messenger RNA was developed to study the regulation of PA synthesis and the genetic elements involved in it. Poly(A)-containing RNA from HEp-3, a PA-rich tumor of human origin, was found to direct the synthesis of a new proteolytic activity when microinjected into Xenopus oocytes. Newly synthesized protease can be detected within a few hours after microinjection of minute quantities of unfractionated mRNA. The new enzymatic activity is indistinguishable from human PA: it is absolutely dependent on human plasminogen; it is neutralized by serum raised against urokinase, the human urinary PA; and it comigrates with urokinase and HEp-3 PA in gel electrophoresis, exhibiting a molecular weight of 60,000.
AB - Induction of synthesis of the protease plasminogen activator (PA) by hormones, oncogenic viruses and tumor promoters occurs at the transcription level. A novel bioassay for PA messenger RNA was developed to study the regulation of PA synthesis and the genetic elements involved in it. Poly(A)-containing RNA from HEp-3, a PA-rich tumor of human origin, was found to direct the synthesis of a new proteolytic activity when microinjected into Xenopus oocytes. Newly synthesized protease can be detected within a few hours after microinjection of minute quantities of unfractionated mRNA. The new enzymatic activity is indistinguishable from human PA: it is absolutely dependent on human plasminogen; it is neutralized by serum raised against urokinase, the human urinary PA; and it comigrates with urokinase and HEp-3 PA in gel electrophoresis, exhibiting a molecular weight of 60,000.
UR - http://www.scopus.com/inward/record.url?scp=0019852667&partnerID=8YFLogxK
U2 - 10.1093/nar/9.14.3355
DO - 10.1093/nar/9.14.3355
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C2 - 6169005
AN - SCOPUS:0019852667
SN - 0305-1048
VL - 9
SP - 3355
EP - 3363
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 14
ER -