Microperoxidase-11 functionalized electrodes: An active monolayer interface for the electrocatalyzed reduction of CoII-protoporphyrin IX reconstituted myoglobin and for the generation of integrated protein electrodes for bioelectrocatalyzed hydrogenation of acetylenes

Vered Heleg-Shabtai, Eugenii Katz, Shlomo Levi, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Microperoxidase-11, MP-11, is assembled as a monolayer on an Au electrode. The resulting MP-11 monolayer electrode mediates the electrocatalyzed reduction of CoII-protoporphyrin IX reconstituted myoglobin, CoII-Mb. The electrocatalyzed reduction of CoII-Mb proceeds via the formation of a complex between the MP-11 monolayer and CoII-Mb. The association constant of the complex formed between MP-11 and CoII-Mb is Ka = 1.6 × 105 M-1. Cross-linking of the complex formed between CoII-Mb and the MP-11 monolayer with glutaric dialdehyde yields a stable integrated electrocatalytic electrode for the stereospecific hydrogenation of acetylenedicarboxylic acid to maleic acid (current yield is ca. 80%). The electrocatalyzed hydrogenation of acetylenedicarboxylic acid reveals an isotope effect, kH/kD ≈ 2.7, suggesting the insertion of the substrate to a CoIII-H species in the rate-limiting step of the hydrogenation process.

Original languageEnglish
Pages (from-to)2645-2651
Number of pages7
JournalJournal of the Chemical Society. Perkin Transactions 2
Issue number12
DOIs
StatePublished - Dec 1997

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