TY - JOUR
T1 - Microperoxidase-11 functionalized electrodes
T2 - An active monolayer interface for the electrocatalyzed reduction of CoII-protoporphyrin IX reconstituted myoglobin and for the generation of integrated protein electrodes for bioelectrocatalyzed hydrogenation of acetylenes
AU - Heleg-Shabtai, Vered
AU - Katz, Eugenii
AU - Levi, Shlomo
AU - Willner, Itamar
PY - 1997/12
Y1 - 1997/12
N2 - Microperoxidase-11, MP-11, is assembled as a monolayer on an Au electrode. The resulting MP-11 monolayer electrode mediates the electrocatalyzed reduction of CoII-protoporphyrin IX reconstituted myoglobin, CoII-Mb. The electrocatalyzed reduction of CoII-Mb proceeds via the formation of a complex between the MP-11 monolayer and CoII-Mb. The association constant of the complex formed between MP-11 and CoII-Mb is Ka = 1.6 × 105 M-1. Cross-linking of the complex formed between CoII-Mb and the MP-11 monolayer with glutaric dialdehyde yields a stable integrated electrocatalytic electrode for the stereospecific hydrogenation of acetylenedicarboxylic acid to maleic acid (current yield is ca. 80%). The electrocatalyzed hydrogenation of acetylenedicarboxylic acid reveals an isotope effect, kH/kD ≈ 2.7, suggesting the insertion of the substrate to a CoIII-H species in the rate-limiting step of the hydrogenation process.
AB - Microperoxidase-11, MP-11, is assembled as a monolayer on an Au electrode. The resulting MP-11 monolayer electrode mediates the electrocatalyzed reduction of CoII-protoporphyrin IX reconstituted myoglobin, CoII-Mb. The electrocatalyzed reduction of CoII-Mb proceeds via the formation of a complex between the MP-11 monolayer and CoII-Mb. The association constant of the complex formed between MP-11 and CoII-Mb is Ka = 1.6 × 105 M-1. Cross-linking of the complex formed between CoII-Mb and the MP-11 monolayer with glutaric dialdehyde yields a stable integrated electrocatalytic electrode for the stereospecific hydrogenation of acetylenedicarboxylic acid to maleic acid (current yield is ca. 80%). The electrocatalyzed hydrogenation of acetylenedicarboxylic acid reveals an isotope effect, kH/kD ≈ 2.7, suggesting the insertion of the substrate to a CoIII-H species in the rate-limiting step of the hydrogenation process.
UR - http://www.scopus.com/inward/record.url?scp=0010352864&partnerID=8YFLogxK
U2 - 10.1039/a704711h
DO - 10.1039/a704711h
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AN - SCOPUS:0010352864
SN - 0300-9580
SP - 2645
EP - 2651
JO - Journal of the Chemical Society. Perkin Transactions 2
JF - Journal of the Chemical Society. Perkin Transactions 2
IS - 12
ER -