TY - JOUR
T1 - Microsatellite instability and p53 mutations in pediatric secondary malignant neoplasms
AU - Gafanovich, Ana
AU - Ramu, Nili
AU - Krichevsky, Svetlana
AU - Pe'er, Jakob
AU - Amir, Gail
AU - Ben-Yehuda, Dina
PY - 1999
Y1 - 1999
N2 - BACKGROUND. The past decade has witnessed a growing frequency of therapy-related secondary tumors. The authors studied nine children with secondary malignancies. The primary tumors were bilateral retinoblastoma, neuroblastoma, brain tumor, Wilms' tumor, colon adenocarcinoma, and Hodgkin's disease. The secondary tumors were osteosarcoma at the site of previous radiotherapy, myelodysplastic syndrome, acute myelocytic leukemia, glioblastoma, thyroid carcinoma, and B-cell lymphoma. METHODS. DNA was extracted from the primary and secondary tumors and analyzed for genetic alterations in the p53 gene and in 7 separate microsatellites. RESULTS. The authors found p53 mutations in 7 patients, loss of heterozygosity in 1 patient, and both mutation and loss of heterozygosity in 1 patient. Mutations were demonstrated in the primary tumors only in two patients and in the secondary tumors only in three patients. Two patients had a mutation in both the primary and the secondary tumor; in both patients the two mutations were in different exons. Microsatellite instability (MIN) was identified in five to seven loci in the secondary tumors of all patients. CONCLUSIONS. The observed MIN is compatible with the presence of a mutator phenotype that predisposes these children to the development of secondary malignancies.
AB - BACKGROUND. The past decade has witnessed a growing frequency of therapy-related secondary tumors. The authors studied nine children with secondary malignancies. The primary tumors were bilateral retinoblastoma, neuroblastoma, brain tumor, Wilms' tumor, colon adenocarcinoma, and Hodgkin's disease. The secondary tumors were osteosarcoma at the site of previous radiotherapy, myelodysplastic syndrome, acute myelocytic leukemia, glioblastoma, thyroid carcinoma, and B-cell lymphoma. METHODS. DNA was extracted from the primary and secondary tumors and analyzed for genetic alterations in the p53 gene and in 7 separate microsatellites. RESULTS. The authors found p53 mutations in 7 patients, loss of heterozygosity in 1 patient, and both mutation and loss of heterozygosity in 1 patient. Mutations were demonstrated in the primary tumors only in two patients and in the secondary tumors only in three patients. Two patients had a mutation in both the primary and the secondary tumor; in both patients the two mutations were in different exons. Microsatellite instability (MIN) was identified in five to seven loci in the secondary tumors of all patients. CONCLUSIONS. The observed MIN is compatible with the presence of a mutator phenotype that predisposes these children to the development of secondary malignancies.
KW - Microsatellite instability
KW - Pediatric secondary malignant neoplasms
KW - Therapy-related tumors
KW - p53 mutations
UR - http://www.scopus.com/inward/record.url?scp=0032942498&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-0142(19990115)85:2<504::AID-CNCR32>3.0.CO;2-C
DO - 10.1002/(SICI)1097-0142(19990115)85:2<504::AID-CNCR32>3.0.CO;2-C
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C2 - 10023722
AN - SCOPUS:0032942498
SN - 0008-543X
VL - 85
SP - 504
EP - 510
JO - Cancer
JF - Cancer
IS - 2
ER -