TY - JOUR
T1 - Microviscosity of togavirus membranes studied by fluorescence depolarization
T2 - influence of envelope proteins and the host cell
AU - Moore, N. F.
AU - Barenholz, Y.
AU - Wagner, R. R.
PY - 1976
Y1 - 1976
N2 - The microviscosities of the hydrophobic regions of the membranes of intact Semliki forest and Sindbis viruses grown on BHK 21 cells, of liposomes derived from the extracted viral lipids, and of protease treated virions were measured by fluorescence depolarization using the fluorescence probe 1,6 diphenyl 1,3,5 hexatriene. The intact virus membranes were found to have a higher microviscosity than did virus derived liposomes, indicating that viral envelope proteins contribute to microviscosity. However, protease treated virus, devoid of protruding spikes but with residual lipophilic peptide tails, was found to have a microviscosity more similar to that of the intact virus than to that of protein free liposomes. Sindbis virus grown in BHK 21 cells at 37 C had a much higher microviscosity than did Sindbis virus grown on Aedes albopictus cells at 22 C. Sindbis virus grown in A. albopictus and BHK 21 cells also gave higher microviscosity values than did the intact host cells. These data indicate that both the virion proteins and the cellular lipids selected during viral growth and maturation contribute to the increased microviscosity of togavirus membranes.
AB - The microviscosities of the hydrophobic regions of the membranes of intact Semliki forest and Sindbis viruses grown on BHK 21 cells, of liposomes derived from the extracted viral lipids, and of protease treated virions were measured by fluorescence depolarization using the fluorescence probe 1,6 diphenyl 1,3,5 hexatriene. The intact virus membranes were found to have a higher microviscosity than did virus derived liposomes, indicating that viral envelope proteins contribute to microviscosity. However, protease treated virus, devoid of protruding spikes but with residual lipophilic peptide tails, was found to have a microviscosity more similar to that of the intact virus than to that of protein free liposomes. Sindbis virus grown in BHK 21 cells at 37 C had a much higher microviscosity than did Sindbis virus grown on Aedes albopictus cells at 22 C. Sindbis virus grown in A. albopictus and BHK 21 cells also gave higher microviscosity values than did the intact host cells. These data indicate that both the virion proteins and the cellular lipids selected during viral growth and maturation contribute to the increased microviscosity of togavirus membranes.
UR - http://www.scopus.com/inward/record.url?scp=0017117756&partnerID=8YFLogxK
U2 - 10.1128/jvi.19.1.126-135.1976
DO - 10.1128/jvi.19.1.126-135.1976
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C2 - 985887
AN - SCOPUS:0017117756
SN - 0022-538X
VL - 19
SP - 126
EP - 135
JO - Journal of Virology
JF - Journal of Virology
IS - 1
ER -