Mimicking Horseradish Peroxidase and NADH Peroxidase by Heterogeneous Cu2+-Modified Graphene Oxide Nanoparticles

Shan Wang, Rémi Cazelles, Wei Ching Liao, Margarita Vázquez-González, Amani Zoabi, Raed Abu-Reziq, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

181 Scopus citations

Abstract

Cu2+-ion-modified graphene oxide nanoparticles, Cu2+-GO NPs, act as a heterogeneous catalyst mimicking functions of horseradish peroxidase, HRP, and of NADH peroxidase. The Cu2+-GO NPs catalyze the oxidation of dopamine to aminochrome by H2O2 and catalyze the generation of chemiluminescence in the presence of luminol and H2O2. The Cu2+-GO NPs provide an active material for the chemiluminescence detection of H2O2 and allow the probing of the activity of H2O2-generating oxidases and the detection of their substrates. This is exemplified with detecting glucose by the aerobic oxidation of glucose by glucose oxidase and the Cu2+-GO NP-stimulated chemiluminescence intensity generated by the H2O2 product. Similarly, the Cu2+-GO NPs catalyze the H2O2 oxidation of NADH to the biologically active NAD+ cofactor.

Original languageAmerican English
Pages (from-to)2043-2048
Number of pages6
JournalNano Letters
Volume17
Issue number3
DOIs
StatePublished - 8 Mar 2017

Bibliographical note

Publisher Copyright:
© 2017 American Chemical Society.

Keywords

  • Catalysis
  • biocatalysis
  • chemiluminescence
  • dopamine
  • nanotechnology

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