Minwuia thermotolerans gen. nov., sp. nov., a marine bacterium forming a deep branch in the alphaproteobacteria, and proposal of minwuiaceae fam. nov. and minwuiales ord. nov.

Cong Sun, Lin Xu, Xiao Yun Yu, Zhe Zhao, Yue Hong Wu, Aharon Oren, Chun Sheng Wang, Xue Wei Xu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Two Gram-stain-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped bacteria, designated as SY3-15 T and SY3-13, were isolated from a seawater sample of the South China Sea. Colonies were 0.5-1.0mm in diameter, smooth, circular, convex and translucent after growth on marine agar at 37 °C for 3 days. The strains were found to grow at 20-50 °C (optimum, 42 °C), pH 6.0-8.5 (optimum, pH 6.5-7.5) and with 0.5-6.0% (w/v) NaCl (optimum, 1.5-2.0 %). Chemotaxonomic analysis showed the sole respiratory quinone to be ubiquinone-10, the major fatty acids (>10 %) were C 16 : 0 3-OH, C 19 : 0 cyclo ω9c, C 18 : 1 3-OH and summed feature 8 (C 18 : 1 ω7c and/or C 18 : 1 ω6c), and the polar lipids were phosphatidylglycerol, two unidentified aminolipids and three unidentified lipids. The DNA G+C content was 67.2-67.4 mol% calculated by genome. The 16S rRNA gene sequences of strains SY3-15 T and SY3-13 were identical and related to the genus Lutibaculum with a similarity of 92.1 %. The 16S rRNA gene phylogenetic trees reconstructed with neighbour-joining, maximum-parsimony and minimum-evolution methods showed that the strains constituted a deep and separated branch from other families of Alphaproteobacteria, and the phylogenetic trees based on concatenated 163 protein sequences from genome sequences showed that the clade in which strains SY3-15 T and SY3-13 located was separated from the clade of the other orders of Alphaproteobacteria, indicating it may represent a novel family of a novel order. Based on their phenotypic properties and their phylogenetic distinctiveness, we propose strains SY3-15 T (=MCCC 1K03467 T =KCTC 62335 T ) and SY3-13 (=MCCC 1K03466=KCTC 62329) to represent a novel species of a novel genus with the name Minwuia thermotolerans gen. nov., sp. nov., and we propose Minwuiaceae fam. nov. and Minwuiales ord. nov. with Minwuia as the type genus.

Original languageAmerican English
Article number003073
Pages (from-to)3856-3862
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue number12
DOIs
StatePublished - Dec 2018

Bibliographical note

Publisher Copyright:
© 2018 IUMS.

Keywords

  • Aerobic
  • Alphaproteobacteria
  • Marine
  • Minwuia thermotolerans
  • Minwuiaceae fam. nov.
  • Minwuiales ord. nov.

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