miR-17-92 and miR-106b-25 clusters regulate beta cell mitotic checkpoint and insulin secretion in mice

Amitai D. Mandelbaum, Sharon Kredo-Russo, Danielle Aronowitz, Nadav Myers, Eran Yanowski, Agnes Klochendler, Avital Swisa, Yuval Dor, Eran Hornstein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Aims/hypothesis: Adult beta cells in the pancreas are the sole source of insulin in the body. Beta cell loss or increased demand for insulin impose metabolic challenges because adult beta cells are generally quiescent and infrequently re-enter the cell division cycle. The aim of this study is to test the hypothesis that a family of proto-oncogene microRNAs that includes miR-17-92 and miR-106b-25 clusters regulates beta cell proliferation or function in the adult endocrine pancreas. Methods: To elucidate the role of miR-17-92 and miR-106b-25 clusters in beta cells, we used a conditional miR-17-92/miR-106b-25 knockout mouse model. We employed metabolic assays in vivo and ex vivo, together with advanced microscopy of pancreatic sections, bioinformatics, mass spectrometry and next generation sequencing, to examine potential targets of miR-17-92/miR-106b-25, by which they might regulate beta cell proliferation and function. Results: We demonstrate that miR-17-92/miR-106b-25 regulate the adult beta cell mitotic checkpoint and that miR-17-92/miR-106b-25 deficiency results in reduction in beta cell mass in vivo. Furthermore, we reveal a critical role for miR-17-92/miR-106b-25 in glucose homeostasis and in controlling insulin secretion. We identify protein kinase A as a new relevant molecular pathway downstream of miR-17-92/miR-106b-25 in control of adult beta cell division and glucose homeostasis. Conclusions/interpretation: The study contributes to the understanding of proto-oncogene miRNAs in the normal, untransformed endocrine pancreas and illustrates new genetic means for regulation of beta cell mitosis and function by non-coding RNAs. Data availability: Sequencing data that support the findings of this study have been deposited in GEO with the accession code GSE126516.

Original languageAmerican English
Pages (from-to)1653-1666
Number of pages14
Issue number9
StatePublished - 1 Sep 2019

Bibliographical note

Funding Information:
Funding The work is funded by an ERC consolidator program (617351), Juvenile Diabetes Research Foundation (99-2007-71 and 47-2012-742), European Diabetes Research Programmes (EFSD)/D-Cure young Investigator award and EFSD-Lilly, Yeda-Sela, Yeda-CEO, Y. Leon Benoziyo Institute for Molecular Medicine, Kekst Family Institute for Medical Genetics, David and Fela Shapell Family Center for Genetic Disorders Research, Crown Human Genome Center, Nathan, Shirley, Philip and Charlene Vener New Scientist Fund, Julius and Ray Charlestein Foundation, Fraida Foundation, Wolfson Family Charitable Trust, Adelis Foundation, Merck (UK), Maria Halphen and Estates of Fannie Sherr, Lola Asseof, Lilly Fulop. Hornstein laboratory is supported by Dr. Sydney Brenner. EH is Head of Nella and Leon Benoziyo Center for Neurological Diseases and incumbent of Ira & Gail Mondry Professorial chair. The study sponsor was not involved in the design of the study, the collection, analysis and interpretation of data, writing the report or the decision to submit the report for publication.

Funding Information:
The authors would like to thank Y. Melamed and O. Higfa for veterinary services and husbandry (Weizmann Institute of Science). The authors thank A. Savidor and Y. Levin at the de Botton Institute for Protein Profiling, Weizmann Institute of Science, and O. Ben-Ami of the Crown Genomics Institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, for MS and next generation sequencing, respectively. We thank O. Elhanani, M. Walker, S. Itzkovitz, H. Kaspi, R. Pasvolsky and E. Geron (Weizmann Institute of Science) for insightful comments on the manuscript.

Publisher Copyright:
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature.


  • Beta cells
  • Cell cycle
  • Diabetes
  • GSIS
  • Glucose-stimulated insulin secretion
  • PKA
  • Protein kinase A
  • microRNA


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