MiRNA-320a is less expressed and miRNA-148a more expressed in preterm human milk compared to term human milk

Yaffa Elbaum Shiff, Shimon Reif, Ronella Marom, Kobi Shiff, Ram Reifen, Regina Golan-Gerstl*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Objectives: To investigate whether there is a difference in the profile of miRNAs between human milk (HM) from mothers of preterm versus HM from mothers of full-term infants. Second goal is to assess biological functions or implication related to those differences in miRNAs expression. Methods: Four of the highly expressed miRNAs in milk were detected by qRT-PCR. Milk derived exosomes were incubated with cells. The expression of miRNAs and target gene were detected by qRT-PCR. Results: MiRNA-320 was more highly expressed in the colostrum of fullterm than in preterm HM. The expression of MiRNA-148 was higher in preterm mother's milk than of full-term colostrum. MiRNA-320 and MIRNA-148a expression were upregulated in cells incubated with milk exosomes, which lead to a decrease in their target genes FASN1 and DNMT1 respectivilly. Conclusions: Alterations in miRNAs expression in HM can affect biologic function in infants and may serve as a nutritional therapeutic target.

Original languageAmerican English
Pages (from-to)68-74
Number of pages7
JournalJournal of Functional Foods
Volume57
DOIs
StatePublished - Jun 2019

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Ltd

Keywords

  • Breastfeeding
  • DNMT1
  • Fatty acid synthase
  • Milk
  • Preterm
  • miRNA

Fingerprint

Dive into the research topics of 'MiRNA-320a is less expressed and miRNA-148a more expressed in preterm human milk compared to term human milk'. Together they form a unique fingerprint.

Cite this