Misidentification of Candida dubliniensis isolates with the VITEK MS

Maya Korem; Matan Joel Cohen; Ayelet Michael-Gayego; Dafna Castiel; Marc V. Assous; Sharon Amit

doi:10.1016/j.mycmed.2020.101107

Misidentification of Candida dubliniensis isolates with the VITEK MS

Maya Korem^*
, Matan Joel Cohen
, Ayelet Michael-Gayego
, Dafna Castiel
, Marc V. Assous
, Sharon Amit

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The phylogenetic relatedness of Candida dubliniensis and C. albicans may lead to misidentification of C. dubliniensis and underestimation of its clinical significance. We evaluated the performance of VITEK-MS in identifying C. dubliniensis isolates following growth on different culture media. Correct identification was documented in 98% of the isolates grown on blood agar media whereas only 44% were correctly identified from SDA or CHROMagar. The use of non-manufacturer validated media for identifying C. dubliniensis with VITEK-MS, may result in misidentification of these isolates as C. albicans. This finding calls for reassessing the accuracy of fungal isolates identification in local workflows using non-validated culture media.

Original language	English
Article number	101107
Journal	Journal of Medical Mycology
Volume	31
Issue number	1
DOIs	https://doi.org/10.1016/j.mycmed.2020.101107
State	Published - Mar 2021

Bibliographical note

Publisher Copyright:
© 2020

Keywords

Candida albicans
Candida dubliniensis
CHROMagar
MALDI-TOF MS
VITEK MS

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10.1016/j.mycmed.2020.101107

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title = "Misidentification of Candida dubliniensis isolates with the VITEK MS",

abstract = "The phylogenetic relatedness of Candida dubliniensis and C. albicans may lead to misidentification of C. dubliniensis and underestimation of its clinical significance. We evaluated the performance of VITEK-MS in identifying C. dubliniensis isolates following growth on different culture media. Correct identification was documented in 98\% of the isolates grown on blood agar media whereas only 44\% were correctly identified from SDA or CHROMagar. The use of non-manufacturer validated media for identifying C. dubliniensis with VITEK-MS, may result in misidentification of these isolates as C. albicans. This finding calls for reassessing the accuracy of fungal isolates identification in local workflows using non-validated culture media.",

keywords = "Candida albicans, Candida dubliniensis, CHROMagar, MALDI-TOF MS, VITEK MS",

author = "Maya Korem and Cohen, \{Matan Joel\} and Ayelet Michael-Gayego and Dafna Castiel and Assous, \{Marc V.\} and Sharon Amit",

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doi = "10.1016/j.mycmed.2020.101107",

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AU - Cohen, Matan Joel

AU - Michael-Gayego, Ayelet

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AU - Assous, Marc V.

AU - Amit, Sharon

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AB - The phylogenetic relatedness of Candida dubliniensis and C. albicans may lead to misidentification of C. dubliniensis and underestimation of its clinical significance. We evaluated the performance of VITEK-MS in identifying C. dubliniensis isolates following growth on different culture media. Correct identification was documented in 98% of the isolates grown on blood agar media whereas only 44% were correctly identified from SDA or CHROMagar. The use of non-manufacturer validated media for identifying C. dubliniensis with VITEK-MS, may result in misidentification of these isolates as C. albicans. This finding calls for reassessing the accuracy of fungal isolates identification in local workflows using non-validated culture media.

KW - Candida albicans

KW - Candida dubliniensis

KW - CHROMagar

KW - MALDI-TOF MS

KW - VITEK MS

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ER -

Misidentification of Candida dubliniensis isolates with the VITEK MS

Abstract

Bibliographical note

Keywords

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