TY - JOUR
T1 - Mitigating temporal mismatches in forensic soil microbial profiles
AU - Pasternak, Zohar
AU - Luchibia, Aineah Obed
AU - Matan, Ofra
AU - Dawson, Lorna
AU - Gafny, Ron
AU - Shpitzen, Moshe
AU - Avraham, Shlomit
AU - Jurkevitch, Edouard
N1 - Publisher Copyright:
© 2018, © 2018 Australian Academy of Forensic Sciences.
PY - 2019/11/2
Y1 - 2019/11/2
N2 - Forensic implementation of soil bacterial DNA profiling is limited by the potential for temporal mismatch of DNA profiles, e.g. after storage or seasonal changes. We compared profiles of samples retrieved at one location over 14 years after air-drying, freeze-drying and –80 °C freezing storage. Sample mismatch in freeze-dried and air-dried samples was significant after two years and continued to increase yearly, whereas profiles after –80 °C freezing remained unchanged for many years. In an attempt to mitigate inter-seasonal temporal mismatches, e.g. when months pass between crime and seizure of evidence, soils sampled in winter and summer were exposed to artificial ‘summer’ and ‘winter’ conditions, respectively, and their DNA profiles were compared. Differences were small between soil types, larger between seasons and largest between ‘natural’ and ‘artificial’ seasons. Understanding sources of temporal variations is critical for storage of forensics samples and for developing mitigation procedures that could help overcome these time-induced limitations.
AB - Forensic implementation of soil bacterial DNA profiling is limited by the potential for temporal mismatch of DNA profiles, e.g. after storage or seasonal changes. We compared profiles of samples retrieved at one location over 14 years after air-drying, freeze-drying and –80 °C freezing storage. Sample mismatch in freeze-dried and air-dried samples was significant after two years and continued to increase yearly, whereas profiles after –80 °C freezing remained unchanged for many years. In an attempt to mitigate inter-seasonal temporal mismatches, e.g. when months pass between crime and seizure of evidence, soils sampled in winter and summer were exposed to artificial ‘summer’ and ‘winter’ conditions, respectively, and their DNA profiles were compared. Differences were small between soil types, larger between seasons and largest between ‘natural’ and ‘artificial’ seasons. Understanding sources of temporal variations is critical for storage of forensics samples and for developing mitigation procedures that could help overcome these time-induced limitations.
KW - Soil
KW - TRFLP
KW - bacteria
KW - sample storage
KW - temporal mismatch
UR - http://www.scopus.com/inward/record.url?scp=85044776068&partnerID=8YFLogxK
U2 - 10.1080/00450618.2018.1450897
DO - 10.1080/00450618.2018.1450897
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AN - SCOPUS:85044776068
SN - 0045-0618
VL - 51
SP - 685
EP - 694
JO - Australian Journal of Forensic Sciences
JF - Australian Journal of Forensic Sciences
IS - 6
ER -